细菌益生菌和酵母后益生菌的组合影响线虫的脂肪沉积和生长

IF 2.4 Q3 NUTRITION & DIETETICS
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引用次数: 0

摘要

背景益生菌是活的微生物,对人类健康(包括肥胖症)具有预期的益处。作为一种小型、快速生长的全生物体模型,秀丽隐杆线虫(Caenorhabditis elegans)已被用于评估益生菌对健康的影响,其机制可通过现有的遗传工具进行评估。线虫的研究结果可以提供特定益生菌菌株以及益生菌与益生元和益生元组合对健康相关生理学影响的数据,为选择干预措施进行进一步研究提供依据。我们假设,益生菌与益生元的特定组合既能加快蠕虫的生长发育,又能减少脂肪沉积,这表明它们能更有效地利用营养物质。结果我们的研究结果表明,益生菌植物乳杆菌 ABB S20 与后效益生菌非活性酵母 K. marxianus ABB S8 和 S. boulardii ABB S3 的结合使用会对生长和发育速度以及脂肪沉积产生影响。结论:这些结果表明,利用秀丽隐杆线虫作为模型,可以有效地筛选益生菌、益生元和益后元的组合,从而找到有助于有效利用营养和控制体重的候选益生菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Combinations of bacterial probiotics and yeast postbiotics influence fat deposition and growth in the nematode C. elegans

Background

Probiotics are live microorganisms with intended benefits on human health including obesity. As a small and fast-growing whole organism model, Caenorhabditis elegans has been used to assess the health effects of probiotics where mechanisms can be assessed through available genetic tools. Results from C. elegans can provide data on the effect of specific probiotic strains and combinations with prebiotics and postbiotics on health-related physiology to inform selections of interventions for further study. We hypothesized that specific combinations with prebiotics and postbiotics could both speed up worm development and reduce fat deposition, suggesting they allow for more effective nutrient utilization.

Methods

Here we expose C. elegans to the ABB S20 strain of Lactiplantibacillus plantarum in combination with different prebiotics and postbiotics. We then measure how these affect growth and development speed as well as fat deposition by measuring the time until the appearance of progeny and measuring Oil Red O staining respectively.

Results

Our results show that the combination of probiotic L. plantarum ABB S20 plus the postbiotic inactive yeasts K. marxianus ABB S8 and S. boulardii ABB S3 resulted in fast growth and reduced fat deposition compared to L. plantarum ABB S20 alone.

Conclusion

These results demonstrate the usefulness of C. elegans as a model to efficiently screen though combinations of probiotics, prebiotics and postbiotics to find those that are candidates to help with effective nutrition use and therefore weight management.

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来源期刊
PharmaNutrition
PharmaNutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.70
自引率
3.10%
发文量
33
审稿时长
12 days
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