{"title":"利用甘蔗工业废渣生产高分子量葡聚糖的统计优化、动力学建模和技术经济分析","authors":"","doi":"10.1016/j.biteb.2024.101902","DOIUrl":null,"url":null,"abstract":"<div><p>This study focuses on the transformation of industrial wastes to wealth by utilizing treated sugarcane molasses (TCM) to produce dextran. The fermentation conditions for maximum dextran production were initially optimized using central-composite design in shake-flasks. The highest titer of dextran (60.0 ± 2.0 g/L) was obtained with optimized variables of 150 g/L substrate (TCM), 12.8 g/L yeast extract, 39.8 g/L K<sub>2</sub>HPO<sub>4</sub>, and 48 h fermentation time. Then, the profiles of dextran production, TCM consumption, and microbial growth were fitted by kinetic models to obtain the following kinetic parameters: 0.35 h<sup>−1</sup> maximum specific growth rate (μ<sub>max</sub>), 0.48 g dextran/g substrate yield coefficient (<em>Y</em><sub><em>ps</em></sub>), 0.07 maintenance coefficient (<em>m</em><sub><em>s</em></sub>), and 10.73 g product/g cell growth-associated constant (<em>α</em>). For determining the scale-up factors, the fermentation conditions were replicated in a 3 L fermenter at various stirring speeds (50–250 rpm), and a scale-up strategy based on constant P/V was used to predict the power consumption (1.88–285.51 W) for a pilot-scale of 2000 L working volume fermenter at various stirring speeds (10.8–54 rpm). The dextran produced was characterized using gel permeation chromatography to determine the molecular mass variations (3–4000 kDa) with fermentation conditions. The rheological variations of fermentation broth at different stirring speeds were also studied and related to the molecular mass of the dextran produced. Techno-economic analysis for dextran production explored a gross margin of 22.65 %, a return on investment of 16.80 %, and a pay-back time of 5.95 years.</p></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Statistical optimization, kinetic modeling, and techno-economic analysis for the production of high molecular mass dextran using sugarcane industrial waste-molasses\",\"authors\":\"\",\"doi\":\"10.1016/j.biteb.2024.101902\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study focuses on the transformation of industrial wastes to wealth by utilizing treated sugarcane molasses (TCM) to produce dextran. The fermentation conditions for maximum dextran production were initially optimized using central-composite design in shake-flasks. The highest titer of dextran (60.0 ± 2.0 g/L) was obtained with optimized variables of 150 g/L substrate (TCM), 12.8 g/L yeast extract, 39.8 g/L K<sub>2</sub>HPO<sub>4</sub>, and 48 h fermentation time. Then, the profiles of dextran production, TCM consumption, and microbial growth were fitted by kinetic models to obtain the following kinetic parameters: 0.35 h<sup>−1</sup> maximum specific growth rate (μ<sub>max</sub>), 0.48 g dextran/g substrate yield coefficient (<em>Y</em><sub><em>ps</em></sub>), 0.07 maintenance coefficient (<em>m</em><sub><em>s</em></sub>), and 10.73 g product/g cell growth-associated constant (<em>α</em>). For determining the scale-up factors, the fermentation conditions were replicated in a 3 L fermenter at various stirring speeds (50–250 rpm), and a scale-up strategy based on constant P/V was used to predict the power consumption (1.88–285.51 W) for a pilot-scale of 2000 L working volume fermenter at various stirring speeds (10.8–54 rpm). The dextran produced was characterized using gel permeation chromatography to determine the molecular mass variations (3–4000 kDa) with fermentation conditions. The rheological variations of fermentation broth at different stirring speeds were also studied and related to the molecular mass of the dextran produced. Techno-economic analysis for dextran production explored a gross margin of 22.65 %, a return on investment of 16.80 %, and a pay-back time of 5.95 years.</p></div>\",\"PeriodicalId\":8947,\"journal\":{\"name\":\"Bioresource Technology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589014X24001439\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589014X24001439","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 0
摘要
本研究的重点是利用经过处理的甘蔗糖蜜(TCM)生产葡聚糖,将工业废物转化为财富。在摇瓶中使用中心复合设计法初步优化了最大右旋糖酐产量的发酵条件。在 150 克/升底物(中药)、12.8 克/升酵母提取物、39.8 克/升 K2HPO4 和 48 小时发酵时间的优化变量下,右旋糖酐的滴度最高(60.0 ± 2.0 克/升)。然后,用动力学模型拟合葡聚糖产量、中药消耗量和微生物生长曲线,得到以下动力学参数:0.35 h-1 最大比生长速率(μmax)、0.48 g 右旋糖酐/g 底物产量系数(Yps)、0.07 维持系数(ms)和 10.73 g 产物/g 细胞生长相关常数(α)。为确定放大系数,在 3 L 发酵罐中以不同的搅拌速度(50-250 rpm)重复发酵条件,并采用基于恒定 P/V 的放大策略来预测 2000 L 工作容积的中试规模发酵罐在不同搅拌速度(10.8-54 rpm)下的耗电量(1.88-285.51 W)。使用凝胶渗透色谱法对生产的葡聚糖进行了表征,以确定其分子质量随发酵条件的变化(3-4000 kDa)。此外,还研究了不同搅拌速度下发酵液的流变学变化,并将其与所产生的葡聚糖的分子质量联系起来。葡聚糖生产的技术经济分析显示,毛利率为 22.65%,投资回报率为 16.80%,投资回收期为 5.95 年。
Statistical optimization, kinetic modeling, and techno-economic analysis for the production of high molecular mass dextran using sugarcane industrial waste-molasses
This study focuses on the transformation of industrial wastes to wealth by utilizing treated sugarcane molasses (TCM) to produce dextran. The fermentation conditions for maximum dextran production were initially optimized using central-composite design in shake-flasks. The highest titer of dextran (60.0 ± 2.0 g/L) was obtained with optimized variables of 150 g/L substrate (TCM), 12.8 g/L yeast extract, 39.8 g/L K2HPO4, and 48 h fermentation time. Then, the profiles of dextran production, TCM consumption, and microbial growth were fitted by kinetic models to obtain the following kinetic parameters: 0.35 h−1 maximum specific growth rate (μmax), 0.48 g dextran/g substrate yield coefficient (Yps), 0.07 maintenance coefficient (ms), and 10.73 g product/g cell growth-associated constant (α). For determining the scale-up factors, the fermentation conditions were replicated in a 3 L fermenter at various stirring speeds (50–250 rpm), and a scale-up strategy based on constant P/V was used to predict the power consumption (1.88–285.51 W) for a pilot-scale of 2000 L working volume fermenter at various stirring speeds (10.8–54 rpm). The dextran produced was characterized using gel permeation chromatography to determine the molecular mass variations (3–4000 kDa) with fermentation conditions. The rheological variations of fermentation broth at different stirring speeds were also studied and related to the molecular mass of the dextran produced. Techno-economic analysis for dextran production explored a gross margin of 22.65 %, a return on investment of 16.80 %, and a pay-back time of 5.95 years.