Yue Qi , Chen Wang , Haoyu Lang , Yueyi Wang , Xiaofei Wang , Hao Zheng , Yuan Lu
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引用次数: 0
摘要
陶瓷鼻疽蝇（Nosema ceranae）是一种寄生于蜜蜂肠道并在其中繁殖的寄生虫，已成为全球养蜂业的严重威胁。RNA 干扰（RNAi）技术可通过抑制 N. ceranae 中的硫氧还蛋白还原酶（TrxR）来抑制 N. ceranae 的生长。然而，由于dsRNA在蜜蜂体内容易降解，合适的载体是限制RNAi应用的原因之一。脂质体作为一种由脂质双分子层组成的囊泡，是一种很好的核酸递送载体，但在蜜蜂中缺乏研究。本研究利用脂质体递送双链 RNA（dsRNA）dsTrxR，触发 RNAi，从而抑制蜜蜂中神经鞘螨的生长。与裸露的dsTrxR相比，脂质体-dsTrxR减少了中肠中的N. ceranae数量，并部分恢复了中肠形态，而不影响蜜蜂的存活和肠道微生物组成。该研究结果证实脂质体能有效保护dsRNA进入蜜蜂肠道，为利用RNAi技术抑制蜜蜂病虫害提供了参考。
Liposome-based RNAi delivery in honeybee for inhibiting parasite Nosema ceranae
Nosema ceranae, a parasite that parasitizes and reproduces in the gut of honeybees, has become a serious threat to the global apiculture industry. RNA interference (RNAi) technology can be used to inhibit N. ceranae growth by targeting silencing the thioredoxin reductase (TrxR) in N. ceranae. However, suitable carriers are one of the reasons limiting the application of RNAi due to the easy degradation of dsRNA in honeybees. As a vesicle composed of a lipid bilayer, liposomes are a good carrier for nucleic acid delivery, but studies in honeybees are lacking. In this study, liposomes were used for double-stranded RNA (dsRNA) dsTrxR delivery triggering RNAi to inhibit the N. ceranae growth in honeybees. Compared to naked dsTrxR, liposome-dsTrxR reduced N. ceranae numbers in the midgut and partially restored midgut morphology without affecting bee survival and gut microbial composition. The results of this study confirmed that liposomes could effectively protect dsRNA from entering the honeybee gut and provide a reference for using RNAi technology to suppress honeybee pests and diseases.
期刊介绍：
Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.
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