来自 Alternaria sp. Strain HU 的蓝色和黄色酵母酶：特征描述与磁性纳米颗粒固定化

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Journal of Fungi Pub Date : 2024-08-08 DOI:10.3390/jof10080559

Ingrida Radveikienė, Regina Vidžiūnaitė, Rolandas Meškys, Vida Časaitė

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引用次数: 0

摘要

漆酶是一种重要而有价值的酶，在生物技术应用方面具有巨大潜力。本研究纯化并鉴定了来自 Alternaria sp.每种同工酶的分子质量约为 66 kDa。LacHU1 漆酶呈黄色，没有典型的蓝色氧化酶光谱，而 LacHU2 呈蓝色，有特征性的吸收光谱。LacHU1 对大多数底物的催化效率高于 LacHU2 漆酶。两种同工酶都能有效氧化类黄酮。在磁性纳米粒子上成功固定了交替孢霉属漆酶。与游离的漆酶相比，固定化漆酶的热稳定性增加，最佳 pH 值也偏向碱性。来自 Alternaria sp. HU 的裂解酶的潜在应用领域是棉花中黄酮类化合物的氧化或水处理过程。

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Blue and Yellow Laccases from Alternaria sp. Strain HU: Characterization and Immobilization on Magnetic Nanoparticles

Laccases are important and valuable enzymes with a great potential for biotechnological applications. In this study, two novel laccases, LacHU1 and LacHU2, from Alternaria sp. HU have been purified and characterized. The molecular mass of each isoenzyme was ~66 kDa. LacHU1 laccases was yellow and had no typical blue oxidase spectra and LacHU2 had a blue color and characteristic absorption spectra. The catalytic efficiency of LacHU1 for most substrates was higher than that of LacHU2 laccase. Both isoenzymes effectively oxidize flavonoids. Alternaria sp. laccases were successfully immobilized on magnetic nanoparticles. The thermostability of immobilized laccases increased and optimal pH shifted to more alkaline compared to the free laccases. Potential applications of laccases from Alternaria sp. HU are in the oxidation of flavonoids in cotton or in water treatment processes.

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.