饮用水处理和分配系统中携带抗生素耐药基因的 1 类整合子及其基因盒的普遍性

IF 6.1 2区 环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL
Shengnan Liu, Zihan Li, Yan Shen, Shuyu Jia, Peng Liu, Xu-Xiang Zhang
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引用次数: 0

摘要

1 类整合子是参与抗生素耐药基因(ARG)环境传播的重要移动遗传元件。然而,人们对饮用水处理和分配过程中 1 类整合子和基因盒的多样性和丰度的了解仍然有限。在本研究中,我们结合了依赖培养和不依赖培养的方法,旨在揭示饮用水处理和分配系统中 1 类整合子的普遍性。此外,我们还采用了纳米孔测序法来描述 1 类整合子相关基因盒所携带的 ARGs 的多样性和排列方式。在从饮用水中分离出的 208 株菌株中,共有 42 株为 intI1 阳性,与 intI1 阴性的菌株相比,它们往往具有多重耐药性。原水中 intI1 的绝对丰度平均为 1.15 × 109 拷贝/升,在液氯消毒后显著降低了 99.9% 以上。此外,纳米孔测序显示,饮用水中的 1 类整合子相关基因盒携带 51 种亚型的 ARGs,主要对氨基糖苷类药物和三甲氧苄氨嘧啶类药物产生耐药性。处理过程,特别是液氯消毒,减少了大部分基因盒携带的 ARGs,但一些 ARG 亚型在处理和分布过程中仍然存在,如 aac(6′)-II、aadA 和 dfrB2。抗生素耐药基因盒阵列∣aac(6′)-II∣arr∣最常被检测到,尤其是在氯化水中。这项研究强调,饮用水是整合子介导的 ARGs 转移的潜在 "蓄水池",表明 1 类整合子中的抗性基因盒对健康的危害亟待关注。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Prevalence of class 1 integron and its gene cassettes carrying antibiotic resistance genes in drinking water treatment and distribution systems

Prevalence of class 1 integron and its gene cassettes carrying antibiotic resistance genes in drinking water treatment and distribution systems

Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes (ARGs). However, knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited. In this study, we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods. Further, we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes. A total of 42 isolates were intI1-positive among the 208 strains isolated from drinking water, which tended to confer multi-drug resistance compared with intI1-negative isolates. The absolute abundance of the intI1 average 1.15 × 109 copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chlorine disinfection. Furthermore, nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water, mainly conferring resistance to aminoglycosides and trimethoprim. The treatment processes, especially liquid chlorine disinfection, reduced most of the ARGs carried by gene cassettes, though some of the ARG subtypes persisted along the treatment and distribution like aac(6′)-II, aadA, and dfrB2. The antibiotic resistance gene cassette array ∣aac(6′)-IIarr∣ was most frequently detected, especially in the chlorinated water. This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer, indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.

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来源期刊
Frontiers of Environmental Science & Engineering
Frontiers of Environmental Science & Engineering ENGINEERING, ENVIRONMENTAL-ENVIRONMENTAL SCIENCES
CiteScore
10.90
自引率
12.50%
发文量
988
审稿时长
6.1 months
期刊介绍: Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines. FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.
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