{"title":"饮用水处理和分配系统中携带抗生素耐药基因的 1 类整合子及其基因盒的普遍性","authors":"Shengnan Liu, Zihan Li, Yan Shen, Shuyu Jia, Peng Liu, Xu-Xiang Zhang","doi":"10.1007/s11783-024-1886-2","DOIUrl":null,"url":null,"abstract":"<p>Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes (ARGs). However, knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited. In this study, we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods. Further, we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes. A total of 42 isolates were <i>intI</i>1-positive among the 208 strains isolated from drinking water, which tended to confer multi-drug resistance compared with <i>intI</i>1-negative isolates. The absolute abundance of the <i>intI</i>1 average 1.15 × 10<sup>9</sup> copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chlorine disinfection. Furthermore, nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water, mainly conferring resistance to aminoglycosides and trimethoprim. The treatment processes, especially liquid chlorine disinfection, reduced most of the ARGs carried by gene cassettes, though some of the ARG subtypes persisted along the treatment and distribution like <i>aac(6′)-II</i>, <i>aadA</i>, and <i>dfrB2</i>. The antibiotic resistance gene cassette array ∣<i>aac(6′)-II</i>∣<i>arr</i>∣ was most frequently detected, especially in the chlorinated water. This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer, indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.\n</p>","PeriodicalId":12720,"journal":{"name":"Frontiers of Environmental Science & Engineering","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of class 1 integron and its gene cassettes carrying antibiotic resistance genes in drinking water treatment and distribution systems\",\"authors\":\"Shengnan Liu, Zihan Li, Yan Shen, Shuyu Jia, Peng Liu, Xu-Xiang Zhang\",\"doi\":\"10.1007/s11783-024-1886-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes (ARGs). However, knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited. In this study, we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods. Further, we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes. A total of 42 isolates were <i>intI</i>1-positive among the 208 strains isolated from drinking water, which tended to confer multi-drug resistance compared with <i>intI</i>1-negative isolates. The absolute abundance of the <i>intI</i>1 average 1.15 × 10<sup>9</sup> copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chlorine disinfection. Furthermore, nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water, mainly conferring resistance to aminoglycosides and trimethoprim. The treatment processes, especially liquid chlorine disinfection, reduced most of the ARGs carried by gene cassettes, though some of the ARG subtypes persisted along the treatment and distribution like <i>aac(6′)-II</i>, <i>aadA</i>, and <i>dfrB2</i>. The antibiotic resistance gene cassette array ∣<i>aac(6′)-II</i>∣<i>arr</i>∣ was most frequently detected, especially in the chlorinated water. This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer, indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.\\n</p>\",\"PeriodicalId\":12720,\"journal\":{\"name\":\"Frontiers of Environmental Science & Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers of Environmental Science & Engineering\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s11783-024-1886-2\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers of Environmental Science & Engineering","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s11783-024-1886-2","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Prevalence of class 1 integron and its gene cassettes carrying antibiotic resistance genes in drinking water treatment and distribution systems
Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes (ARGs). However, knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited. In this study, we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods. Further, we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes. A total of 42 isolates were intI1-positive among the 208 strains isolated from drinking water, which tended to confer multi-drug resistance compared with intI1-negative isolates. The absolute abundance of the intI1 average 1.15 × 109 copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chlorine disinfection. Furthermore, nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water, mainly conferring resistance to aminoglycosides and trimethoprim. The treatment processes, especially liquid chlorine disinfection, reduced most of the ARGs carried by gene cassettes, though some of the ARG subtypes persisted along the treatment and distribution like aac(6′)-II, aadA, and dfrB2. The antibiotic resistance gene cassette array ∣aac(6′)-II∣arr∣ was most frequently detected, especially in the chlorinated water. This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer, indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.
期刊介绍:
Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines.
FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.