新型螺衍生物 4-氨基-1-氮杂螺[4.5]癸-3-烯-2-酮对大蜡蛾的毒理学评估

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
Mogeda M. Abdel Hafez, Jamin Ali, Khalid Ali Khan, Hamed A. Ghramh, Reda M. Fekry, Rehab M. El‐Gendy
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引用次数: 0

摘要

大蜡蛾(Galleria mellonella L.)对养蜂业构成严重威胁,不仅对蜡质造成不利影响,还可能成为病媒。目前迫切需要开发创新、有效的控制策略,以应对这种害虫带来的挑战,确保养蜂业的可持续发展。螺衍生物化合物是一类新型的环境安全化合物,具有很强的杀虫特性。例如,它们对蚜虫和粉虱等吸食汁液的昆虫具有很高的药效。然而,它们对咀嚼昆虫的作用尚未得到很好的研究。在本研究中,我们假设 4-氨基-1-氮杂螺[4.5]癸-3-烯-2-酮(4A1AD)--一种螺衍生物化合物--将对 G. mellonella 幼虫有效。为了验证这一假说,我们在第四龄幼虫身上添加了不同浓度的 4A1AD 进行浸泡和喂食生物测定,并记录了死亡率、生化参数(包括总可溶性蛋白含量和酶活性)以及处理后出现的解剖异常。结果表明,在两种生物测定中,随着浓度和处理时间的增加,幼虫死亡率明显增加。此外,还观察到蛋白质含量明显降低,酚氧化酶活性增加,α 和 β 酯酶活性发生变化。扫描电子显微镜结果显示,幼虫的角质层、螺旋开口和腿出现异常。这些研究结果证明了 4A1AD 的功效,表明它有可能替代传统杀虫剂,促进可持续养蜂业的发展。此外,它们还为养蜂环境中有效害虫管理新策略的开发做出了宝贵贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxicological assessment of a novel spiro‐derivative, 4‐amino‐1‐azaspiro[4.5]dec‐3‐en‐2‐one for the greater wax moth, Galleria mellonella
The greater wax moth, Galleria mellonella L., poses a substantial threat to apiculture, adversely impacting wax quality and potentially acting as a disease vector. There is an urgent need to develop innovative and effective control strategies to address the challenges posed by this pest and ensure the sustainability of the apiculture industry. Spiro‐derivative compounds represent a novel class of environmentally safe compounds with high insecticidal properties. For example, they have demonstrated high efficacy against sap‐sucking insects such as aphids and whiteflies. However, their effect on chewing insects has not yet been well explored. In this study, we hypothesized that 4‐amino‐1‐azaspiro[4.5]dec‐3‐en‐2‐one (4A1AD), a spiro‐derivative compound, would exhibit efficacy against G. mellonella larvae. To test this hypothesis, we conducted dipping and feeding bioassays, incorporating varying concentrations of 4A1AD on fourth instar G. mellonella larvae, and recorded mortality, biochemical parameters including total soluble protein content and enzyme activities, and anatomical abnormalities resulting from treatment. Results revealed a significant increase in larval mortality with increased concentrations and treatment durations across both bioassays. Additionally, a significant decrease in protein content and an increase in phenol‐oxidase activity were observed with alterations in α and β‐esterase activities. Scanning electron microscope findings revealed abnormalities in larval cuticle, spiracular openings and legs. These findings demonstrated the efficacy of 4A1AD, indicating a potential alternative to conventional insecticides in promoting sustainable apiculture practices. Moreover, they provide valuable contributions to the development of novel strategies for effective pest management in apicultural settings.
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来源期刊
CiteScore
3.40
自引率
5.30%
发文量
132
审稿时长
6 months
期刊介绍: The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.
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