覆盆子酮对异丙肾上腺素诱导的 Wistar 大鼠心肌肥大的保护作用

IF 0.8 4区 医学 Q4 CHEMISTRY, MEDICINAL
Vasim Khan, Sumit Sharma, Aamir khan, Uma Bhandari, Syed Ehtaishamul Haque
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引用次数: 0

摘要

覆盆子酮(RK)是从蔷薇科植物茜草(Rubus idaeus)中提取的一种活性成分,传统上用于治疗糖尿病、高血压等。本研究旨在评估覆盆子酮对异丙肾上腺素(ISO)诱导的 Wistar 大鼠心肌肥厚的保护作用。大鼠被任意分为六组,每天接受覆盆子酮(100 和 200 毫克/千克)、非诺贝特(80 毫克/千克)和普萘洛尔(10 毫克/千克)以及 ISO(3 毫克/千克,皮下注射)治疗,连续 28 天。最后一次给药 24 小时后,采集动物的血清和心脏组织样本,并检测其血液动力学(血压、心率]、生化指标(CK-MB、LDH、肌钙蛋白-T、PPAR-α、脂蛋白 C III、c 反应蛋白、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白在进行重量分析的同时,还进行了组织病理学和免疫组织化学(Caspase-3、核因子-κB、肿瘤坏死因子-α)分析。施用 ISO 会明显改变心脏的完整性和生理机能,而施用 RK(100 和 200 毫克/千克)则会明显避免这些变化。研究还发现,这些结果与标准药物非诺贝特和普萘洛尔的效果相当。通过组织病理学和免疫组化分析对心脏形态的评估进一步验证了这些结果。因此,本研究表明,剂量为 100 和 200 毫克/千克的 RK 对 ISO 诱导的 Wistar 大鼠心肌肥厚具有剂量依赖性,可减少炎症、氧化应激和血脂异常,这可能是由于 RK 对 PPAR-α 受体亚型具有激动作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protective Effects of Raspberry Ketone against Isoproterenol-Induced Cardiac Hypertrophy in Wistar Rats

Protective Effects of Raspberry Ketone against Isoproterenol-Induced Cardiac Hypertrophy in Wistar Rats

Raspberry ketone (RK) is an active constituent obtained from Rubus idaeus (Rosaceae), which has been traditionally used against diabetes, hypertension, etc. The objective of this study was to assess the cardioprotective effect of RK against isoproterenol (ISO)-induced cardiac hypertrophy in Wistar rats. Rats were arbitrarily divided into six groups and were treated daily with raspberry ketone (100 and 200 mg/kg), fenofibrate (80 mg/kg) and propranolol (10 mg/kg) along with ISO (3 mg/kg, subcutaneously) for 28 days. Twenty-four hours after the last dose administration, serum and heart tissue samples from the animals were taken and their hemodynamic (blood pressure, heart rate], biochemical (CK-MB, LDH, troponin-T, PPAR-α, apolipoprotein C III, c-reactive protein, total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, very-low-density lipoprotein, malondialdehyde, reduced glutathione, superoxide dismutase, catalase, Na+-K+-ATPase, total antioxidant capacity, and nitric oxide), histopathological, and immunohistochemical (caspase-3, nuclear factor-κB, tumor necrosis factor-α) analysis was performed along with gravimetric analysis. Administration of ISO significantly altered the cardiac integrity and physiology and these changes were significantly averted by the administration of RK (100 and 200 mg/kg). These results were also found to be comparable with those of the standard drugs, fenofibrate and propranolol. The assessment of cardiac morphology by histopathological and immunohistochemical analysis further validated these results. This study thus demonstrated that RK at doses of 100 and 200 mg/kg showed a dose-dependent decrease in inflammation, oxidative stress, and dyslipidemia against ISO-induced cardiac hypertrophy in Wistar rats, which could be due to agonistic action of RK at PPAR-α receptor subtype.

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来源期刊
Pharmaceutical Chemistry Journal
Pharmaceutical Chemistry Journal CHEMISTRY, MEDICINAL-PHARMACOLOGY & PHARMACY
CiteScore
1.30
自引率
22.20%
发文量
226
审稿时长
3-8 weeks
期刊介绍: Pharmaceutical Chemistry Journal is a monthly publication devoted to scientific and technical research on the creation of new drugs and the improvement of manufacturing technology of drugs and intermediates. International contributors cover the entire spectrum of new drug research, including: methods of synthesis; results of pharmacological, toxicological, and biochemical studies; investigation of structure - activity relationships in prediction of new compounds; methods and technical facilities used; and problems associated with the development of ecologically safe and economically feasible methods of industrial production. In addition, analytical reviews of the international literature in the field provide coverage of the most recent developments around the world. Pharmaceutical Chemistry Journal is a translation of the Russian journal Khimiko-Farmatsevticheskii Zhurnal. The Russian Volume Year is published in English from April. All articles are peer-reviewed.
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