Nader Amini, Kazhal Naderi, Abbas Ahmadi, Kambiz Hassanzadeh, Mohammad-Nazir Menbari, Mohammad Abdi, Ebrahim Ghaderi and Mohammad-Rezgar Zarehbin
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Based on the electrochemical results, the interaction of chlorpromazine (CPZ), a widely used antipsychotic tranquillizer, with the allosteric protein, hemoglobin, has been studied. First, four groups of six female rats weighing 400–450 g were selected. The rats were injected with different concentrations of chlorpromazine over a 3-week period, and the concentrations of hemoglobin, methemoglobin, red blood cells (RBCs), and hematocrit (HCT) were analyzed in the blood of each rat. After injection of different concentrations of the drug, the amount of hemoglobin) as a source of Fe (II)) decreased, but the amount of methemoglobin (as a source of Fe (III) increased. In addition, UV spectroscopic measurements in the range of 200–700 nm indicate the conversion of hemoglobin to methemoglobin in chlorpromazine-treated rats compared to the normal sample, and there was a direct relationship between the increasing methemoglobin concentration of chlorpromazine. Furthermore, the amount of RBC and HCT was measured. The results showed that RBC (21.05%–56.52%) and HCT (10.04%–53.19%) decreased. 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引用次数: 0
摘要
本研究在掺硼金刚石(BDD)电极上通过循环伏安法研究了盐酸氯丙嗪(CPZ)水溶液在铁(II)存在下的电化学性质。结果表明发生了 EC′ 反应机制,即电化学生成的 CPZ 物种(阳离子自由基)被 Fe (II) 还原成 CPZ 母体,而 Fe (II) 被氧化成 Fe (III)。检测限、灵敏度和动态浓度范围分别为 2.8 μM、0.0188 μA μM-1 和 10-166 μM。根据电化学结果,研究了氯丙嗪(CPZ)这种广泛使用的抗精神病镇定剂与异构蛋白血红蛋白的相互作用。首先,研究人员选取了四组体重为 400-450 克的六只雌性大鼠。给大鼠注射不同浓度的氯丙嗪,为期 3 周,分析每只大鼠血液中血红蛋白、高铁血红蛋白、红细胞(RBC)和血细胞比容(HCT)的浓度。注射不同浓度的药物后,作为铁(II)来源的血红蛋白量减少,但作为铁(III)来源的高铁血红蛋白量增加。此外,200-700 纳米范围内的紫外光谱测量结果表明,与正常样本相比,氯丙嗪处理的大鼠血红蛋白转化为高铁血红蛋白,而高铁血红蛋白浓度的增加与氯丙嗪有直接关系。此外,还测量了 RBC 和 HCT 的数量。结果显示,RBC(21.05%-56.52%)和 HCT(10.04%-53.19%)均有所下降。最后,本研究根据电化学结果证明了 CPZ 对大鼠血液中血红蛋白铁影响的新机制。
Electrochemical oxidation of Fe (II) using chlorpromazine drug at boron-doped diamond electrode: application to in vivo mechanism study interaction of chlorpromazine on hemoglobin iron and evaluation of some biomolecules
In this study, the electrochemical properties of aqueous chlorpromazine hydrochloride (CPZ) in the presence of Fe (II) were investigated by cyclic voltammetry at a boron-doped diamond (BDD) electrode. The results showed that an EC′ reaction mechanism occurs, where electrochemically generated CPZ species (cation radical) are reduced by Fe (II) back to the parent CPZ, and Fe (II) is oxidized to Fe (III). The detection limit, sensitivity, and dynamic concentration ranges were 2.8 μM, 0.0188 μA μM−1 and 10–166 μM. Based on the electrochemical results, the interaction of chlorpromazine (CPZ), a widely used antipsychotic tranquillizer, with the allosteric protein, hemoglobin, has been studied. First, four groups of six female rats weighing 400–450 g were selected. The rats were injected with different concentrations of chlorpromazine over a 3-week period, and the concentrations of hemoglobin, methemoglobin, red blood cells (RBCs), and hematocrit (HCT) were analyzed in the blood of each rat. After injection of different concentrations of the drug, the amount of hemoglobin) as a source of Fe (II)) decreased, but the amount of methemoglobin (as a source of Fe (III) increased. In addition, UV spectroscopic measurements in the range of 200–700 nm indicate the conversion of hemoglobin to methemoglobin in chlorpromazine-treated rats compared to the normal sample, and there was a direct relationship between the increasing methemoglobin concentration of chlorpromazine. Furthermore, the amount of RBC and HCT was measured. The results showed that RBC (21.05%–56.52%) and HCT (10.04%–53.19%) decreased. Finally, this study demonstrates a new mechanism for the effects of CPZ on hemoglobin iron in rat blood based on electrochemical results.
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