Qiang Wu, Yiluo Zhong, Fei Wang, Yuwei Chen, Wenxiang Zou
{"title":"洪泛平原湖泊(中国鄱阳湖)沉积物反硝化细菌群落在雨季、中旱季和旱季的变化","authors":"Qiang Wu, Yiluo Zhong, Fei Wang, Yuwei Chen, Wenxiang Zou","doi":"10.1007/s10750-024-05666-4","DOIUrl":null,"url":null,"abstract":"<p>To date, seasonal dynamics of the denitrifying bacterial community in floodplain lakes is unknown. Here, we investigated the alpha diversity, abundance and composition of the sediment <i>nirS</i>-type denitrifying bacteria in Poyang Lake during the wet, mid-dry and dry seasons using Illumina MiSeq sequencing and quantitative polymerase chain reaction assay. The results showed that only a small part of the total operational taxonomic units (OTUs) were shared by the three seasons. OTU richness, Chao richness estimator and Shannon index exhibited higher values in the wet season than in the dry season. Some regular patterns of variation in the composition of the <i>nirS</i>-type denitrifying community at three classification levels (phylum, class and genus) were found among seasons. Community structures of <i>nirS</i>-type denitrifying bacteria were significantly different across seasons. In addition, the abundance of <i>nirS</i> gene showed no seasonal pattern. Total nitrogen, total phosphorous and pH were the most significant factors elucidating the compositional variation of the <i>nirS</i>-type denitrifying community. This study provided a new evidence for the ecological effects of seasonal water level fluctuations and improved our understanding of the nitrogen removal processes in floodplain lakes.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"11 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Variation of the sediment denitrifying bacterial community in a floodplain lake (Poyang Lake, China) during the wet, mid-dry and dry seasons\",\"authors\":\"Qiang Wu, Yiluo Zhong, Fei Wang, Yuwei Chen, Wenxiang Zou\",\"doi\":\"10.1007/s10750-024-05666-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>To date, seasonal dynamics of the denitrifying bacterial community in floodplain lakes is unknown. Here, we investigated the alpha diversity, abundance and composition of the sediment <i>nirS</i>-type denitrifying bacteria in Poyang Lake during the wet, mid-dry and dry seasons using Illumina MiSeq sequencing and quantitative polymerase chain reaction assay. The results showed that only a small part of the total operational taxonomic units (OTUs) were shared by the three seasons. OTU richness, Chao richness estimator and Shannon index exhibited higher values in the wet season than in the dry season. Some regular patterns of variation in the composition of the <i>nirS</i>-type denitrifying community at three classification levels (phylum, class and genus) were found among seasons. Community structures of <i>nirS</i>-type denitrifying bacteria were significantly different across seasons. In addition, the abundance of <i>nirS</i> gene showed no seasonal pattern. Total nitrogen, total phosphorous and pH were the most significant factors elucidating the compositional variation of the <i>nirS</i>-type denitrifying community. This study provided a new evidence for the ecological effects of seasonal water level fluctuations and improved our understanding of the nitrogen removal processes in floodplain lakes.</p>\",\"PeriodicalId\":13147,\"journal\":{\"name\":\"Hydrobiologia\",\"volume\":\"11 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hydrobiologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10750-024-05666-4\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hydrobiologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10750-024-05666-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
Variation of the sediment denitrifying bacterial community in a floodplain lake (Poyang Lake, China) during the wet, mid-dry and dry seasons
To date, seasonal dynamics of the denitrifying bacterial community in floodplain lakes is unknown. Here, we investigated the alpha diversity, abundance and composition of the sediment nirS-type denitrifying bacteria in Poyang Lake during the wet, mid-dry and dry seasons using Illumina MiSeq sequencing and quantitative polymerase chain reaction assay. The results showed that only a small part of the total operational taxonomic units (OTUs) were shared by the three seasons. OTU richness, Chao richness estimator and Shannon index exhibited higher values in the wet season than in the dry season. Some regular patterns of variation in the composition of the nirS-type denitrifying community at three classification levels (phylum, class and genus) were found among seasons. Community structures of nirS-type denitrifying bacteria were significantly different across seasons. In addition, the abundance of nirS gene showed no seasonal pattern. Total nitrogen, total phosphorous and pH were the most significant factors elucidating the compositional variation of the nirS-type denitrifying community. This study provided a new evidence for the ecological effects of seasonal water level fluctuations and improved our understanding of the nitrogen removal processes in floodplain lakes.
期刊介绍:
Hydrobiologia publishes original research, reviews and opinions regarding the biology of all aquatic environments, including the impact of human activities. We welcome molecular-, organism-, community- and ecosystem-level studies in contributions dealing with limnology and oceanography, including systematics and aquatic ecology. Hypothesis-driven experimental research is preferred, but also theoretical papers or articles with large descriptive content will be considered, provided they are made relevant to a broad hydrobiological audience. Applied aspects will be considered if firmly embedded in an ecological context.