多用途树(Neolamarckia cadamba)芽鳞包裹叶片的体外嫩枝再生系统

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Buye Li, Qingmin Que, Chunmei Li, Wei Zhou, Xiaoyang Chen, Lifeng Zhang, Kunpeng Du, Qixian Xu, Wenping Chen, Ming Zhong, Zhensen Zeng, Xiaoling Huang, Kunxi Ouyang
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引用次数: 0

摘要

卡当巴树(Neolamarckia cadamba,N. cadamba)是一种常绿树种,因其生长迅速、木材特性显著以及在医药、饲养和景观方面的重要价值而闻名。为了克隆出具有优良基因型的卡当巴树个体,我们成功地建立了以芽鳞包裹的叶片为外植体的植株再生方案。叶片的最佳灭菌方法是先用 0.1%氯化汞(HgCl2)处理 1 分钟,然后再在添加了 3.0 mg/L 噻螨酮(TDZ)、0.1 mg/L 2-4 二氯苯氧乙酸(2-4D)、0.05 mg/L α-萘乙酸(NAA)和 1 mL/L 植物防腐剂混合物(PPM)的 Murashige and Skoog's 培养基(MS)上培养,以诱导胼胝体。含有 1 mL/L PPM 的培养基可有效抑制外植体污染,且不会对最终的叶片胼胝体诱导率产生不利影响。在上述培养基上培养的叶片可诱导出三种类型的胼胝体。其中,在添加了 1.5 毫克/升 6-苄基氨基嘌呤(6-BA)和 0.05 毫克/升 NAA 的 MS 培养基上,只有Ⅱ型胼胝体能分化出不定芽,这种胼胝体呈绿色,有节,颗粒块少,诱导率为 78.89%,每个胼胝体的不定芽数为 11.67 个。在添加了 1.0 毫克/升 6-BA 和 0.05 毫克/升吲哚-3-丁酸(IBA)的 MS 培养基上,不定芽开始增殖,增殖系数为 3.37。在添加了 0.05 mg/L NAA 和 0.05 mg/L IBA 的 MS 培养基中,微芽生根。该再生方案可用于大田繁殖和大规模生产具有相同基因型的幼苗,可作为卡丹巴牛(N. cadamba)的优良个体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In vitro shoot regeneration system from leaves wrapped by bud scales of a multipurpose tree (Neolamarckia cadamba)

In vitro shoot regeneration system from leaves wrapped by bud scales of a multipurpose tree (Neolamarckia cadamba)

Neolamarckia cadamba (N. cadamba) is an evergreen tree species known for its rapid growth, remarkable wood properties, and significant value in medicine, feeding, and landscape. In order to clone a N. cadamba individual with excellent genotype, a plant regeneration protocol was successfully established with leaves wrapped by bud scales as explants. The optimal sterilization method for the leaves was 0.1% Mercury Chloride (HgCl2) treatment for 1 min before culturing on Murashige and Skoog’s medium (MS) supplemented with 3.0 mg/L Thidiazuron (TDZ), 0.1 mg/L 2–4 Dichlorophenoxyacetic acid (2-4D), 0.05 mg/L α-Naphthaleneacetic acid (NAA) and 1 mL/L Plant Preservative Mixture (PPM) to induce calluses. The medium containing 1 mL/L PPM could effectively inhibit explant contamination without an unfavorable impact on the final induction rate of callus from the leaves. Three types of calluses were induced from the leaves cultured on the above medium. Among them, only the Type II callus, which was green and nodular, had few particle masses, could differentiate into adventitious shoots on the MS medium supplemented with 1.5 mg/L 6–Benzylaminopurine (6-BA) and 0.05 mg/L NAA, with the induction rate of 78.89% and adventitious shoot number per callus of 11.67. The adventitious shoots were proliferated on the MS medium supplemented with 1.0 mg/L 6-BA and 0.05 mg/L Indole-3- butyric acid (IBA) with the proliferation coefficient of 3.37. And the micro-shoots developed roots in the MS medium supplemented with 0.05 mg/L NAA and 0.05 mg/L IBA. The regeneration protocol can be used in the propagation and large scale production of seedlings with the same genotype as an excellent individual of N. cadamba in the field.

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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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