Renan Carrari-Santos, Rosana Gobbi Vettorazzi, Tadeu dos Reis Oliveira, Mateus Santana Rodrigues, Edinaldo de Oliveira Alves Sena, Jurandi Gonçalves de Oliveira, Vanildo Silveira, Claudete Santa-Catarina
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The use of microporous membranes plays an important role in the accumulation of proteins related to photosynthetic activity process, such as ruBisCO_large domain-containing protein; ribulose bisphosphate carboxylase small chain; ribulose bisphosphate carboxylase small chain protein; and chlorophyll a-b binding protein, chloroplastic. Conversely, the decreased accumulation of proteins associated with amino acid synthesis linked to ethylene biosynthesis, such as aspartate aminotransferase, in shoots grown with microporous membranes was related to the lower level of ethylene than in shoots grown without membranes. The microporous membrane promoted an increase in total free PAs and putrescine contents in shoots, while higher CO<sub>2</sub> levels were detected in shoots grown without a microporous membrane. This is the first study showing the effects of promoting gas exchange on shoot development in <i>D. nigra</i>, in addition to exploring the interaction of protein accumulation with ethylene. 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引用次数: 0
摘要
使用带微孔膜的盖子可以通过促进气体交换(包括乙烯交换)来增强离体植物的生长。我们的目的是评估微孔膜、外植体类型和培养基对黑木耳(Dalbergia nigra)芽体外发育以及蛋白质谱、多胺(PAs)、乙烯和二氧化碳水平的影响。共鉴定出 465 种蛋白质,其中 148 种为差异积累蛋白质,与不含微孔膜的芽相比,在含微孔膜的密封盖培养瓶中生长的芽增加了 73 种蛋白质,减少了 75 种蛋白质。微孔膜的使用对光合作用过程中相关蛋白质的积累起到了重要作用,如含 ruBisCO_large 结构域的蛋白质、核酮糖二磷酸羧化酶小链、核酮糖二磷酸羧化酶小链蛋白质和叶绿素 a-b 结合蛋白、叶绿体等。相反,与乙烯生物合成相关的氨基酸合成蛋白(如天门冬氨酸氨基转移酶)在有微孔膜生长的嫩枝中积累减少,这与乙烯水平低于无膜生长的嫩枝有关。微孔膜促进了幼芽中总游离 PAs 和腐胺含量的增加,而在无微孔膜的幼芽中则检测到较高的 CO2 含量。这是首次研究显示促进气体交换对黑叶麦冬嫩枝发育的影响,此外还探讨了蛋白质积累与乙烯的相互作用。这项研究可以提高人们对濒危物种黑木耳繁殖系统的认识。
Enhancing in vitro growth and development of Dalbergia nigra shoots using microporous membranes: impacts on gas exchange, protein profile, ethylene, and polyamine levels
The use of lids with microporous membranes can enhance in vitro plant growth by promoting gas exchange, including ethylene exchange. We aimed to evaluate the influence of the microporous membrane, explant type, and culture medium on the in vitro development of Dalbergia nigra shoots, as well as on the protein profile, polyamines (PAs), ethylene and CO2 levels. A total of 465 proteins were identified, of which 148 were differentially accumulated proteins, being 73 proteins up- and 75 down-accumulated in shoots grown in sealed-lid culture flasks containing microporous membranes compared to those grown without. The use of microporous membranes plays an important role in the accumulation of proteins related to photosynthetic activity process, such as ruBisCO_large domain-containing protein; ribulose bisphosphate carboxylase small chain; ribulose bisphosphate carboxylase small chain protein; and chlorophyll a-b binding protein, chloroplastic. Conversely, the decreased accumulation of proteins associated with amino acid synthesis linked to ethylene biosynthesis, such as aspartate aminotransferase, in shoots grown with microporous membranes was related to the lower level of ethylene than in shoots grown without membranes. The microporous membrane promoted an increase in total free PAs and putrescine contents in shoots, while higher CO2 levels were detected in shoots grown without a microporous membrane. This is the first study showing the effects of promoting gas exchange on shoot development in D. nigra, in addition to exploring the interaction of protein accumulation with ethylene. This research can improve the understanding of propagation systems in D. nigra, an endangered species.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.