Bison sex matters: the potential of proteomic tooth enamel analysis for determination of ancient human subsistence strategies

Hunting strategies in the Paleolithic period represent a significant and complex challenge, influencing the lifestyles of early human populations dependent on the seasonal activity of herds. Varying seasonally, a composition of a herd caused changes in the speed of animals movements, their aggressiveness, hides and the fatness and flavor of their meat. The morphological criteria for determining grazer sex and age have long been established in zooarchaeology. However, many traits associated with bison sex are poorly defined for archeozoological collections due to the fragmented and scattered nature of the bone remains. The objective of presented study was to demonstrate the potential utility of proteomic tooth enamel analysis detecting the AmelX and AmelY peptide fragments for prey-sex determination in cases where morphological criteria did not work. In the study, the tooth enamel samples from 18 animals were analyzed, including eight modern specimens of known sex, and ten Pleistocene specimens of unknown sex from four different Paleolithic sites. A blind test conducted for the modern collection yielded a 100% accuracy. This outcome prompted the development of the criteria for sex determination in the bison based on proteomic analysis of tooth enamel.