{"title":"用于诊断慢性肾病的快速、高选择性 ECL 肌酐传感器","authors":"Hosein Afshary and Mandana Amiri","doi":"10.1039/D4SD00165F","DOIUrl":null,"url":null,"abstract":"<p >Monitoring of creatinine in human fluid has attracted considerable attention owing to the potential for diagnosis of chronic kidney disease. However, the detection of creatinine has been difficult owing to its electrochemical and optical inertness. In this approach, a highly selective and sensitive electrochemiluminescence (ECL) strategy based on homogeneous carbon quantum dots (CQDs) for the detection of creatinine was introduced. A copper(<small>II</small>) picrate complex was added at the surface of electrode to improve the selectivity of the sensor significantly by the formation of a Janovsky complex. A multi-pulse amperometric technique was applied as a very fast and reliable method for quantitative determination of creatinine. The calibration curve was acquired with a linear range from 1.0 × 10<small><sup>−8</sup></small> to 1 × 10<small><sup>−5</sup></small> M with a low detection limit of 8.7 × 10<small><sup>−9</sup></small> M. The proposed creatinine sensing platform is experimentally very simple and shows high selectivity with a broad linear range of detection. Furthermore, the presented method can determine creatinine in real samples with excellent recoveries.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1562-1570"},"PeriodicalIF":3.5000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00165f?page=search","citationCount":"0","resultStr":"{\"title\":\"A fast and highly selective ECL creatinine sensor for diagnosis of chronic kidney disease†\",\"authors\":\"Hosein Afshary and Mandana Amiri\",\"doi\":\"10.1039/D4SD00165F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Monitoring of creatinine in human fluid has attracted considerable attention owing to the potential for diagnosis of chronic kidney disease. However, the detection of creatinine has been difficult owing to its electrochemical and optical inertness. In this approach, a highly selective and sensitive electrochemiluminescence (ECL) strategy based on homogeneous carbon quantum dots (CQDs) for the detection of creatinine was introduced. A copper(<small>II</small>) picrate complex was added at the surface of electrode to improve the selectivity of the sensor significantly by the formation of a Janovsky complex. A multi-pulse amperometric technique was applied as a very fast and reliable method for quantitative determination of creatinine. The calibration curve was acquired with a linear range from 1.0 × 10<small><sup>−8</sup></small> to 1 × 10<small><sup>−5</sup></small> M with a low detection limit of 8.7 × 10<small><sup>−9</sup></small> M. The proposed creatinine sensing platform is experimentally very simple and shows high selectivity with a broad linear range of detection. Furthermore, the presented method can determine creatinine in real samples with excellent recoveries.</p>\",\"PeriodicalId\":74786,\"journal\":{\"name\":\"Sensors & diagnostics\",\"volume\":\" 9\",\"pages\":\" 1562-1570\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00165f?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors & diagnostics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00165f\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00165f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
摘要
由于肌酐可用于诊断慢性肾脏疾病,因此对人体液中肌酐的监测备受关注。然而,由于肌酐具有电化学和光学惰性,其检测一直很困难。在这种方法中,引入了一种基于均质碳量子点(CQDs)的高选择性、高灵敏度电化学发光(ECL)策略来检测肌酐。在电极表面添加了吡啶甲酸铜 (II) 复合物,通过形成 Janovsky 复合物显著提高了传感器的选择性。多脉冲安培计技术是一种快速可靠的肌酐定量测定方法。所提出的肌酐传感平台在实验上非常简单,而且具有高选择性和较宽的线性检测范围。此外,所提出的方法还能测定真实样品中的肌酐,且回收率极高。
A fast and highly selective ECL creatinine sensor for diagnosis of chronic kidney disease†
Monitoring of creatinine in human fluid has attracted considerable attention owing to the potential for diagnosis of chronic kidney disease. However, the detection of creatinine has been difficult owing to its electrochemical and optical inertness. In this approach, a highly selective and sensitive electrochemiluminescence (ECL) strategy based on homogeneous carbon quantum dots (CQDs) for the detection of creatinine was introduced. A copper(II) picrate complex was added at the surface of electrode to improve the selectivity of the sensor significantly by the formation of a Janovsky complex. A multi-pulse amperometric technique was applied as a very fast and reliable method for quantitative determination of creatinine. The calibration curve was acquired with a linear range from 1.0 × 10−8 to 1 × 10−5 M with a low detection limit of 8.7 × 10−9 M. The proposed creatinine sensing platform is experimentally very simple and shows high selectivity with a broad linear range of detection. Furthermore, the presented method can determine creatinine in real samples with excellent recoveries.