利用新型色聚体灵敏检测 17β 雌二醇的竞争性辣根过氧化物酶标记色聚体分析法

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Qiuyi Cheng and Qiang Zhao
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引用次数: 0

摘要

17β-雌二醇(E2)是典型的内分泌干扰化合物(EDCs)之一,在促进生长和调节人体内分泌系统平衡方面发挥着重要作用。由于暴露于 E2 会通过与雌激素受体结合干扰内分泌系统,因此 E2 污染会对环境和健康造成危害。开发灵敏的 E2 检测方法势在必行。在此,我们利用一种新报道的高亲和性 DNA 类似物作为亲和配体,开发了一种检测 E2 的竞争性酶联检测法。抗 E2 合体的互补 DNA(cDNA)被连接到微孔板上。适配体探针上标记有辣根过氧化物酶(HRP)。在没有 E2 的情况下,HRP 标记的适配体被 cDNA 捕获,HRP 将底物催化成产物,产生吸光度信号或化学发光信号。在 E2 存在的情况下,E2 与适配体结合,导致 HRP 标记的适配体从微孔板中移出,信号减弱。在吸光度分析模式下,E2 的检测限为 0.2 nmol/L,动态范围为 0.2 nmol/L 至 20 μmol/L。在化学发光分析模式下,该方法可定量检测 50 pmol/L 的 E2,动态范围为 50 pmol/L 至 50 μmol/L。该方法还能检测湖泊水样中添加的E2,显示了实际应用的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of 17β-estradiol with a new aptamer†

Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of 17β-estradiol with a new aptamer†

17β-Estradiol (E2) is one of the typical endocrine-disrupting compounds (EDCs), which plays a major role in facilitating the growth and regulating the balance of the human endocrine system. E2 contamination can cause environmental and health risks as E2 exposure can interfere with the endocrine system by binding to estrogen receptors. It is imperative to develop sensitive methods for E2 detection. Herein we developed a competitive enzyme-linked aptamer assay for E2 detection by using a newly reported high-affinity DNA aptamer as an affinity ligand. The complementary DNA (cDNA) of the anti-E2 aptamer is conjugated on a microplate. Horseradish peroxidase (HRP) is labeled on the aptamer probe. In the absence of E2, HRP-labeled aptamer is captured by cDNA, and HRP catalyzes the substrate into a product, generating an absorbance signal or chemiluminescence signal. In the presence of E2, E2 binds with the aptamer, causing displacement of HRP-labeled aptamer from the microplate and a decrease in signals. In absorbance-analysis mode, the detection limit of E2 reached 0.2 nmol L−1 with a dynamic range from 0.2 nmol L−1 to 20 μmol L−1. In chemiluminescenceanalysis mode, this method enabled the quantification of E2 at 50 pmol L−1, with a dynamic range from 50 pmol L−1 to 50 μmol L−1. This method could also detect E2 spiked in lake water samples, showing promise in practical applications.

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CiteScore
2.30
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