利用高性能抗体吸收聚苯乙烯凝集颗粒检测环境污染水源中的致病性钩端螺旋体

IF 2.7 4区 材料科学 Q3 CHEMISTRY, PHYSICAL
Patcharapan Suwannin, Duangporn Polpanich, Noureddine Lebaz, Kween Saimuang, Jirawan Jindakaew, Pramuan Tangboriboonrat, Kulachart Jangpatarapongsa, Abdelhamid Elaissari
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引用次数: 0

摘要

钩端螺旋体病是一种重新出现的细菌性人畜共患病,对人类和动物都有影响,在热带和亚热带地区发病率明显较高。疾病控制、流行病学和监测依赖于 "一体健康 "方法,因为准确的检测可应用于人类、动物和环境。本研究首次尝试开发一种基于乳胶凝集反应的方法来检测致病性 Santarosai 舍曼尼钩端螺旋体血清。在将抗体吸附到聚苯乙烯颗粒上之前,对抗体的血清学活性进行了检测,以获得高滴度的抗体。在 pH 值为 6.8-7.8 的介质中,抗体吸附总量可达 3 mg m-2。将抗体吸附的 PS 与钩端螺旋体培养物孵育 4 分钟后,可观察到颗粒凝集现象,检测限为 1.7 × 102 个钩端螺旋体 mL-1。有趣的是,使用分光光度计观察凝集时,检测限提高了 1000 倍。该检测方法对雪曼尼虫具有高度特异性,与非致病性钩端螺旋体和水生细菌的交叉凝集可忽略不计。在采集的自然水源水样中进行的凝集试验与培养技术有很好的相关性。这种简单快速的钩端螺旋体凝集检测方法可用作环境、人类和动物标本的筛选检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenic Leptospira Detection in Environmental Contaminant Water Sources by Highly Performance Antibody Absorption Polystyrene Agglutinating Particles
Leptospirosis is a re‐emerging bacterial zoonotic disease that affects both humans and animals, with a significantly higher incidence in tropical and sub‐tropical regions. Disease control, epidemiology, and surveillance rely on a One Health approach, as accurate detection can be applied to humans, animals, and the environment. This study represents the first attempt to develop a method for detecting the pathogenic Leptospira santarosai serovar Shermani based on the latex agglutination reaction. The serological activity of the antibody is examined to achieve a high titer of antibody before adsorption onto polystyrene particles. Using a pH medium of 6.8–7.8, total antibody adsorption of up to 3 mg m−2 is achieved. Particle agglutination is observed after incubating the antibody‐adsorbed PS with leptospiral culture for 4 min, revealing a detection limit of 1.7 × 102 leptospires mL−1. Interestingly, the detection limit increased by 1000 times when observing agglutination using spectrophotometer. The test exhibits high specificity with Shermani and shows negligible cross‐agglutination with non‐pathogenic Leptospira and water‐borne bacteria. Agglutination testing in collected water samples from natural sources demonstrates a good correlation with culture technique. This simple and rapid leptospires agglutination detection method can be applied as a screening test in environmental, human, and animal specimens.
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来源期刊
Particle & Particle Systems Characterization
Particle & Particle Systems Characterization 工程技术-材料科学:表征与测试
CiteScore
5.50
自引率
0.00%
发文量
114
审稿时长
3.0 months
期刊介绍: Particle & Particle Systems Characterization is an international, peer-reviewed, interdisciplinary journal focusing on all aspects of particle research. The journal joined the Advanced Materials family of journals in 2013. Particle has an impact factor of 4.194 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)). Topics covered include the synthesis, characterization, and application of particles in a variety of systems and devices. Particle covers nanotubes, fullerenes, micelles and alloy clusters, organic and inorganic materials, polymers, quantum dots, 2D materials, proteins, and other molecular biological systems. Particle Systems include those in biomedicine, catalysis, energy-storage materials, environmental science, micro/nano-electromechanical systems, micro/nano-fluidics, molecular electronics, photonics, sensing, and others. Characterization methods include microscopy, spectroscopy, electrochemical, diffraction, magnetic, and scattering techniques.
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