泛癌分析和单细胞分析发现 FAM110B 是生存和免疫疗法的潜在靶点

IF 3.9 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Frontiers in Molecular Biosciences Pub Date : 2024-08-07 DOI:10.3389/fmolb.2024.1424104

Yuwei Li, Xiaoxi Li, Bihua Wu, Shuangyan Su, Yunpeng Su, Le Guo

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引用次数: 0

摘要

背景：FAM110B 属于序列相似度为 110 的家族（FAM110），位于中心体和有丝分裂纺锤体中。在早期的研究中，FAM110B 与肿瘤细胞的生长有关。FAM110B 在肿瘤微环境以及泛癌症中的功能尚不明确：为了评估 FAM110B 在正常组织和泛癌症组织中的表达变化，我们结合了 TCGA 和 GTEx 数据库。方法：为了评估 FAM110B 在正常组织和泛癌症组织中的表达变化，我们结合了 TCGA 和 GTEx 数据库，并使用 cBioPortal 数据库和 GSCALite 平台研究了 FAM110B 基因组和甲基化改变的变化。Cox回归、Kaplan-Meier和SangerBox被用来研究FAM110B和泛癌症的临床特征和预后。相关性研究的目的是调查免疫相关基因、肿瘤突变负荷、微卫星不稳定性、免疫相关基因和免疫检查点与 FAM110B 表达之间的关联。ESTIMATE、EPIC、QUANTISEQ和MCPCOUNTER方法用于计算FAM110B表达与肿瘤免疫微环境之间的相互作用。单细胞数据库（TISCH 和 CancerSEA）分析了 FAM110B 的免疫渗透和功能。最后，我们通过 GDSC 和 CTRP 数据库评估了 FAM110B 对小分子药物的敏感性：结果：FAM110B的转录和蛋白表达在不同癌症类型中存在显著差异，这对一些肿瘤的预后具有预测价值，包括脑低级胶质瘤（LGG）、胃腺癌（STAD）、胰腺癌（PAAD）等。在肿瘤微环境中，FAM110B的表达水平在一定程度上与免疫细胞浸润、免疫检查点免疫调节基因、肿瘤突变负荷和微卫星脆性有关：这项研究探讨了 FAM110B 作为一种标记物预测泛癌症免疫治疗反应的可能性，为癌症治疗提供了理论依据。

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Pan-cancer analysis and single-cell analysis reveals FAM110B as a potential target for survival and immunotherapy

Background: FAM110B belongs to the family that has a 110 sequence similarity (FAM110) and is located in the centrosome and mitotic spindle. FAM110B has been linked to tumor cell growth in earlier research. Uncertainty exists regarding FAM110B’s function within the tumor microenvironment is unclear as well as pan-cancer.Methods: In order to assess the variation in FAM110B expression within normal and pan-cancer tissues, we combined the TCGA and GTEx databases. The cBioPortal database and the GSCALite platform were used to examine the variation in genome and methylation alteration of FAM110B. Cox regression, Kaplan-Meier, and SangerBox were employed to examine the clinical features and prognosis of FAM110B and pan-cancer. The purpose of the correlational research was to investigate the associations within immunerelated genes, tumor mutation burden, microsatellite instability, immune-related genes, and immunological checkpoints and FAM110B expression. ESTIMATE, EPIC, QUANTISEQ, and MCPCOUNTER methods were used to calculate the interaction among FAM110B expression as well as the tumor immune microenvironment. The immunoinfiltration and function of FAM110B were analyzed by single-cell databases (TISCH and CancerSEA). Finally, we evaluated the sensitivity of FAM110B to small-molecule medications through GDSC and CTRP databases.Results: The transcription and protein expression of FAM110B varies significantly throughout cancer types, and this has predictive value for the prognosis of some tumors; including brain lower grade glioma (LGG), stomach adenocarcinoma (STAD), pancreatic adenocarcinoma (PAAD), etc. In the tumor microenvironment, the expression level of FAM110B was associated with immune cell infiltration, immune checkpoint immune regulatory genes, tumor mutational burden, and microsatellite fragility to a certain extent.Conclusion: This work investigates the possibility of utility of FAM110B as a marker to forecast pan-cancer immunotherapy response, providing a theoretical basis for cancer therapy.

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来源期刊

Frontiers in Molecular Biosciences Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

7.20

自引率

4.00%

发文量

1361

审稿时长

14 weeks

期刊介绍： Much of contemporary investigation in the life sciences is devoted to the molecular-scale understanding of the relationships between genes and the environment — in particular, dynamic alterations in the levels, modifications, and interactions of cellular effectors, including proteins. Frontiers in Molecular Biosciences offers an international publication platform for basic as well as applied research; we encourage contributions spanning both established and emerging areas of biology. To this end, the journal draws from empirical disciplines such as structural biology, enzymology, biochemistry, and biophysics, capitalizing as well on the technological advancements that have enabled metabolomics and proteomics measurements in massively parallel throughput, and the development of robust and innovative computational biology strategies. We also recognize influences from medicine and technology, welcoming studies in molecular genetics, molecular diagnostics and therapeutics, and nanotechnology. Our ultimate objective is the comprehensive illustration of the molecular mechanisms regulating proteins, nucleic acids, carbohydrates, lipids, and small metabolites in organisms across all branches of life. In addition to interesting new findings, techniques, and applications, Frontiers in Molecular Biosciences will consider new testable hypotheses to inspire different perspectives and stimulate scientific dialogue. The integration of in silico, in vitro, and in vivo approaches will benefit endeavors across all domains of the life sciences.