支链氨基酸、丙氨酸与甲状腺功能：基于横截面核磁共振 (NMR) 的 ELSA-Brasil 方法

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-08-06 DOI:10.3390/metabo14080437

Carolina Castro Porto Silva Janovsky, Vandrize Meneghini, William Tebar, Joao Roberto Maciel Martins, José Augusto Sgarbi, Patrícia de Fatima dos Santos Teixeira, Steven R. Jones, Michael J. Blaha, Peter P. Toth, Paulo A. Lotufo, Marcio S. Bittencourt, Raul D. Santos, Itamar S. Santos, Layal Chaker, Isabela M. Bensenor

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引用次数: 0

摘要

尽管必需氨基酸和非必需氨基酸具有共同的代谢作用，但甲状腺功能与这两种氨基酸之间的关系还没有得到充分研究。因此，我们的目的是评估甲状腺功能与普通人群中支链氨基酸（BCAA，即亮氨酸、异亮氨酸和缬氨酸）和丙氨酸水平的关系。我们利用了 ELSA-Brasil 圣保罗研究中心的数据，这是一项基于人群的纵向队列研究。甲状腺参数包括促甲状腺激素（TSH）、游离 T4 和游离 T3 水平以及 FT4:FT3 比率。在全自动 NMR 平台上对 BCAAs 和丙氨酸进行了分析。本次分析包括甲状腺功能正常的参与者以及亚临床甲状腺功能亢进和甲状腺功能减退的参与者。我们使用皮尔逊系数来量化甲状腺相关参数与氨基酸之间的相关性。我们使用线性回归模型来分析甲状腺参数是否与 BCAAs 和丙氨酸水平相关。本研究共纳入 4098 名参与者（51.3 ± 9.0 岁，51.5% 为女性）。在调整最多的模型中，较高的 TSH 水平与较高的丙氨酸水平相关，FT4 水平与异亮氨酸水平成反比，FT3 水平与缬氨酸和亮氨酸呈统计学意义上的正相关，T3：T4 比值与所有氨基酸呈正相关。我们观察到，在所有模型中，亚临床甲状腺机能减退都与异亮氨酸和丙氨酸水平呈正相关，即使经过充分调整也是如此。我们的研究结果突显了亚临床甲状腺机能减退和甲状腺相关参数（包括促甲状腺激素、游离 T4、游离 T3 和 FT4:FT3 比值）与 BCAAs 和丙氨酸的关系。还需要进一步的研究来探索这种关联的机制。这些见解有助于我们了解甲状腺相关参数对 BCAA 和丙氨酸代谢的影响。

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Branched-Chain Amino Acids, Alanine, and Thyroid Function: A Cross-Sectional, Nuclear Magnetic Resonance (NMR)-Based Approach from ELSA-Brasil

The association of thyroid function with essential and non-essential amino acids is understudied, despite their common metabolic roles. Thus, our aim was to evaluate the association of thyroid function with the levels of branched-chain amino acids (BCAAs—leucine, isoleucine, and valine) and of alanine in the general population. We utilized data from the São Paulo research center of ELSA-Brasil, a longitudinal population-based cohort study. Thyroid parameters included thyroid stimulating hormone (TSH), free T4 and free T3 levels, and the FT4:FT3 ratio. BCAAs and alanine were analyzed on a fully automated NMR platform. The current analysis included euthyroid participants and participants with subclinical hyperthyroidism and hypothyroidism. We used Pearson’s coefficient to quantify the correlation between thyroid-related parameters and amino acids. Linear regression models were performed to analyze whether thyroid parameters were associated with BCAAs and alanine levels. We included 4098 participants (51.3 ± 9.0 years old, 51.5% women) in this study. In the most adjusted model, higher levels of TSH were associated with higher levels of alanine, FT4 levels were inversely associated with isoleucine levels, FT3 levels were statistically significant and positively associated with valine and leucine, and the T3:T4 ratio was positively associated with all amino acids. We observed that subclinical hypothyroidism was positively associated with isoleucine and alanine levels in all models, even after full adjustment. Our findings highlight the association of subclinical hypothyroidism and thyroid-related parameters (including TSH, free T4, free T3, and FT4:FT3 ratio) with BCAAs and alanine. Further studies are needed to explore the mechanisms underlying this association. These insights contribute to our understanding of the influence of thyroid-related parameters on BCAA and alanine metabolism.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.