高反式脂肪酸饮食对家兔模型中肾脏、肝脏和心脏相关疾病的影响

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-08-08 DOI:10.3390/metabo14080442

Hammad Ismail, Zaryab Mubashar, Hajra Khan, Zeenat Naveed, Erum Dilshad, Muhammad Zeeshan Bhatti, Sadaf Anwaar, Samreen Saleem, Sabba Mehmood, Abdur Rahman, U. Rashid, Dalia Fouad, M. De Waard, G. Batiha

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引用次数: 0

摘要

反式脂肪酸是加工食品中的特定不饱和脂肪，经过氢化后会导致肝脏疾病，如代谢相关性脂肪肝（MAFLD）以及心血管疾病和慢性肾脏病等。在动物模型中研究了含有反式脂肪酸（薏苡仁酸和油酸）的不同食物样本通过抗氧化酶活性对肝脏、心脏和肾脏的影响。对兔子血清中的肝功能检测（ALT、ALP、AST 和 LDH）、心脏生物标志物水平（CPK、TC、HDL、LDL 和甘油三酯）和肾脏生物标志物水平（血清肌酐、血尿素氮和血尿酸）以及肝组织的组织病理学进行了检测。结果表明，与正常对照组、油酸组和 Kausar Ghee 组相比，Mujahid Ghee 组的这些生物标志物升高幅度更大。穆贾希德酥油组的过氧化物酶、谷胱甘肽、过氧化氢酶、硫代巴比妥酸活性物质和超氧化物歧化酶等抗氧化标志物的浓度较低。高效液相色谱法显示，在所有选定的样品中，穆贾希德酥油的麦饭石酸定量值最高。总之，这项研究表明，最纯形式的麦饭石酸会加重家兔肝脏的 MAFLD，并引发 CVK 和心血管疾病。

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Effects of a High Trans Fatty Acid Diet on Kidney-, Liver-, and Heart-Associated Diseases in a Rabbit Model

Trans fatty acids are specific unsaturated fats found in processed foods that undergo hydrogenation, leading to hepatic disorders such as metabolic-associated fatty liver disease (MAFLD) and conditions like CVD and CKD. The effects of different food samples containing trans fatty acids (elaidic and oleic acid) on the liver, heart, and kidney through antioxidant enzyme activity were investigated in animal models. Liver function tests (ALT, ALP, AST, and LDH), heart biomarker levels (CPK, TC, HDL, LDL, and triglycerides), and kidney biomarker levels (serum creatinine, blood urea nitrogen, and serum uric acid) were examined in serum of rabbits and the histopathology of liver tissues. Results showed that these biomarkers were more elevated in the Mujahid Ghee group than in the normal control, oleic acid, and Kausar Ghee groups. The concentration of antioxidant markers such as peroxidase, glutathione, catalase, thiobarbituric acid reactive substances, and superoxide dismutase were lower in the Mujahid Ghee group. HPLC showed that Mujahid Ghee had the highest quantified value of elaidic acid among all selected samples. Overall, this study demonstrated that elaidic acid in its purest form aggravated MAFLD in rabbit livers and provoked CVK and CVD.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.