Chia-Wei Yeh, Nathaniel Wright, Chelsea Loh, Nabeen Chu, Yadong Wang
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引用次数: 0
摘要
蛋白质和其他生物制剂的可控递送是治疗以前无法治疗的疾病的一种日益增长的媒介。在此，我们报告了一种用于可控递送生长因子和细胞因子的自组装可调囊泡。由肝素和生物相容性聚阳离子 PEAD 组成的包囊构成了囊泡的核心；脂质构成了囊泡的膜。我们称这种囊泡为 "脂溶性囊泡"（LipCo），由于肝素的存在，它对生长因子和细胞因子具有很高的亲和力。LipCo 是一种可调的蛋白质输送载体。囊泡的大小可通过聚合物和盐的浓度来控制。膜功能化使其具有潜在的靶向能力，并可通过冻干长期保存。重要的是，治疗药物的可控递送还能避免对体外处理过的细胞产生高毒性。我们在此报告 LipCo 组装和设计的这些关键原则。
Lipocoacervate, a tunable vesicle for protein delivery
Controlled delivery of proteins and other biologics is a growing medium of therapy for diseases previously untreatable. Here we report a self-assembling, tunable vesicle for the controlled delivery of growth factors and cytokines. Coacervate made of heparin and a biocompatible polycation, PEAD, forms the core of the vesicle; lipids form the membrane of the vesicle. We call this vesicle lipocoacervate (LipCo), which has a high affinity for growth factors and cytokines due to heparin. LipCo is a tunable protein delivery vehicle. The vesicle size is controlled through polymer and salt concentrations. Membrane functionalization enables potential for targeting capabilities with long-term storage through lyophilization. Importantly, the controlled delivery of therapeutics also avoids high toxicity to treated cells in vitro. Here we report on these key principles of LipCo assembly and design.
期刊介绍：
Nano Research is a peer-reviewed, international and interdisciplinary research journal that focuses on all aspects of nanoscience and nanotechnology. It solicits submissions in various topical areas, from basic aspects of nanoscale materials to practical applications. The journal publishes articles on synthesis, characterization, and manipulation of nanomaterials; nanoscale physics, electrical transport, and quantum physics; scanning probe microscopy and spectroscopy; nanofluidics; nanosensors; nanoelectronics and molecular electronics; nano-optics, nano-optoelectronics, and nano-photonics; nanomagnetics; nanobiotechnology and nanomedicine; and nanoscale modeling and simulations. Nano Research offers readers a combination of authoritative and comprehensive Reviews, original cutting-edge research in Communication and Full Paper formats. The journal also prioritizes rapid review to ensure prompt publication.
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