{"title":"通过稳健的比率荧光探针观察药物引起的肝损伤和细菌中的 ClO- 波动。","authors":"","doi":"10.1016/j.saa.2024.124944","DOIUrl":null,"url":null,"abstract":"<div><p>As a type of reactive oxygen species, hypochlorous acid (ClO<sup>-</sup>) plays an important role in sterilization, disinfection and protection in organisms. However, excessive production of ClO<sup>-</sup> is closely related to various diseases. In this work, we have designed a robust ratiometric fluorescent probe, <strong>RDB-ClO</strong>, using the excited-state intramolecular proton transfer (ESIPT) strategy. <strong>RDB-ClO</strong> was achieved by modifying 2-(2-(benzo[d]thiazol-2-yl)-6-(diethylamino)-3-oxo-3H-xanthen-9-yl) benzoic acid (<strong>RDB-OH</strong>) with a 1-naphthoyl chloride group, specifically for the sensitive detection of ClO<sup>-</sup>. In the presence of ClO<sup>-</sup>, <strong>RDB-ClO</strong> demonstrated relatively good performance, showing swift response time (35 s), low detection limit of 5.1 nM and high selectivity towards ClO<sup>-</sup>. Notably, the convenience and accessibility detection of ClO<sup>-</sup> has been implemented using test strip and agarose probe. <strong>RDB-ClO</strong> effectively tracked both endogenous and exogenous ClO<sup>-</sup> in HeLa cells, HepG2 cells and zebrafish. Additionally, it is successfully applied to detect changes of exogenous ClO<sup>-</sup> content in E. coli. and acetaminophen (APAP)-induced liver injury in mice. The development of <strong>RDB-ClO</strong> represents a promising molecular tool for studying the pathogenesis of DILI and biotransformation of ClO<sup>-</sup> in bacteria.</p></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Visualizing ClO- fluctuations in drug-induced liver injury and bacterium via a robust ratiometric fluorescent probe\",\"authors\":\"\",\"doi\":\"10.1016/j.saa.2024.124944\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>As a type of reactive oxygen species, hypochlorous acid (ClO<sup>-</sup>) plays an important role in sterilization, disinfection and protection in organisms. However, excessive production of ClO<sup>-</sup> is closely related to various diseases. In this work, we have designed a robust ratiometric fluorescent probe, <strong>RDB-ClO</strong>, using the excited-state intramolecular proton transfer (ESIPT) strategy. <strong>RDB-ClO</strong> was achieved by modifying 2-(2-(benzo[d]thiazol-2-yl)-6-(diethylamino)-3-oxo-3H-xanthen-9-yl) benzoic acid (<strong>RDB-OH</strong>) with a 1-naphthoyl chloride group, specifically for the sensitive detection of ClO<sup>-</sup>. In the presence of ClO<sup>-</sup>, <strong>RDB-ClO</strong> demonstrated relatively good performance, showing swift response time (35 s), low detection limit of 5.1 nM and high selectivity towards ClO<sup>-</sup>. Notably, the convenience and accessibility detection of ClO<sup>-</sup> has been implemented using test strip and agarose probe. <strong>RDB-ClO</strong> effectively tracked both endogenous and exogenous ClO<sup>-</sup> in HeLa cells, HepG2 cells and zebrafish. Additionally, it is successfully applied to detect changes of exogenous ClO<sup>-</sup> content in E. coli. and acetaminophen (APAP)-induced liver injury in mice. The development of <strong>RDB-ClO</strong> represents a promising molecular tool for studying the pathogenesis of DILI and biotransformation of ClO<sup>-</sup> in bacteria.</p></div>\",\"PeriodicalId\":433,\"journal\":{\"name\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1386142524011107\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142524011107","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
Visualizing ClO- fluctuations in drug-induced liver injury and bacterium via a robust ratiometric fluorescent probe
As a type of reactive oxygen species, hypochlorous acid (ClO-) plays an important role in sterilization, disinfection and protection in organisms. However, excessive production of ClO- is closely related to various diseases. In this work, we have designed a robust ratiometric fluorescent probe, RDB-ClO, using the excited-state intramolecular proton transfer (ESIPT) strategy. RDB-ClO was achieved by modifying 2-(2-(benzo[d]thiazol-2-yl)-6-(diethylamino)-3-oxo-3H-xanthen-9-yl) benzoic acid (RDB-OH) with a 1-naphthoyl chloride group, specifically for the sensitive detection of ClO-. In the presence of ClO-, RDB-ClO demonstrated relatively good performance, showing swift response time (35 s), low detection limit of 5.1 nM and high selectivity towards ClO-. Notably, the convenience and accessibility detection of ClO- has been implemented using test strip and agarose probe. RDB-ClO effectively tracked both endogenous and exogenous ClO- in HeLa cells, HepG2 cells and zebrafish. Additionally, it is successfully applied to detect changes of exogenous ClO- content in E. coli. and acetaminophen (APAP)-induced liver injury in mice. The development of RDB-ClO represents a promising molecular tool for studying the pathogenesis of DILI and biotransformation of ClO- in bacteria.
期刊介绍:
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.