埃塞俄比亚吉吉加市医疗机构接生的新生儿中 B 群链球菌的垂直传播、流行率、相关因素和抗菌药敏感性概况。

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-08-03 eCollection Date: 2024-01-01 DOI:10.1155/2024/5673366
Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle
{"title":"埃塞俄比亚吉吉加市医疗机构接生的新生儿中 B 群链球菌的垂直传播、流行率、相关因素和抗菌药敏感性概况。","authors":"Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle","doi":"10.1155/2024/5673366","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Group B <i>Streptococcus</i> (GBS) colonizes the rectovaginal area of women and vertically transmitted to neonates. This bacterium has been linked to severe neonatal complications including pneumonia, septicemia, and meningitis. GBS shows an increased resistance to commonly used antibiotics. Thus, we assessed the vertical transmission, contributing factors, and antimicrobial resistance patterns of GBS among newborns delivered at selected Hospitals in Jigjiga City.</p><p><strong>Methods: </strong>Hospital-based cross-sectional study was conducted from 1<sup>st</sup> June 2022 to 30th April 2023. A total of 849 pregnant women admitted to delivery wards from two hospitals were screened for GBS colonization. Subsequently, 162 GBS-colonized pregnant women and their newborn babies were included. A semistructured questionnaire and a review of medical records were used to collect the sociodemographic and clinical characteristics of the study participants. Trained nurses collected swab samples from the vaginal-rectal area of pregnant women and the nasal, ear canal, and umbilical areas of newborn babies. Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. All gram-positive cocci, B-hemolytic, and catalase-negative isolates were further identified using Christie-Atkins-Munch-Petersen and a bacitracin test. The modified Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing. Data were analyzed using SPSS version 26. Logistic regression analysis was used to determine the factors associated with vertical transmission of GBS, and statistical significance was set at <i>p</i> values <0.05.</p><p><strong>Result: </strong>The overall vertical transmission rate was 41.4% (67/162). History of preterm labor (Adjusted odds ratio (AOR) = 2.25; 95% CI: 1.11, 4.59), history of urinary tract infection (UTI) at current pregnancy (AOR = 2.25; 95% CI: 1.11, 4.59), and prolonged rupture of membranes greater than 18 hours (AOR = 2.23; 95% CI: 1.13, 4.4) were significantly associated with vertical transmission of GBS from previously colonized mothers to their newborn babies. Regarding GBS antibiotic susceptibility profile, a significant degree of resistance was observed to penicillin (29.9%), tetracycline (22.4%), ampicillin (20.9%), and clindamycin (19.4%).</p><p><strong>Conclusion: </strong>Our study documented a high prevalence of vertical transmission rate of GBS from pregnant women to their babies, with an overall transmission rate of 41.4%. The study identified the presence of antimicrobial-resistant GBS to penicillin, ampicillin, clindamycin, ciprofloxacin, and chloramphenicol. The organism was susceptible to vancomycin, followed by azithromycin, ceftriaxone, and erythromycin. Our study also reported MDR at 13.4%. Based on our findings, there is a need for antenatal culture-based GBS screening, maternal vaccination, and large-scale epidemiological and serotype identification studies to be put into practice in the study area.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2024 ","pages":"5673366"},"PeriodicalIF":2.8000,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316908/pdf/","citationCount":"0","resultStr":"{\"title\":\"Vertical Transmission of Group B <i>Streptococcus</i>, Prevalence, Associated Factors, and Antimicrobial Susceptibility Profile among Newborns Delivered at Health Facilities in Jigjiga City, Ethiopia.\",\"authors\":\"Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle\",\"doi\":\"10.1155/2024/5673366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Group B <i>Streptococcus</i> (GBS) colonizes the rectovaginal area of women and vertically transmitted to neonates. This bacterium has been linked to severe neonatal complications including pneumonia, septicemia, and meningitis. GBS shows an increased resistance to commonly used antibiotics. Thus, we assessed the vertical transmission, contributing factors, and antimicrobial resistance patterns of GBS among newborns delivered at selected Hospitals in Jigjiga City.</p><p><strong>Methods: </strong>Hospital-based cross-sectional study was conducted from 1<sup>st</sup> June 2022 to 30th April 2023. A total of 849 pregnant women admitted to delivery wards from two hospitals were screened for GBS colonization. Subsequently, 162 GBS-colonized pregnant women and their newborn babies were included. A semistructured questionnaire and a review of medical records were used to collect the sociodemographic and clinical characteristics of the study participants. Trained nurses collected swab samples from the vaginal-rectal area of pregnant women and the nasal, ear canal, and umbilical areas of newborn babies. Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. All gram-positive cocci, B-hemolytic, and catalase-negative isolates were further identified using Christie-Atkins-Munch-Petersen and a bacitracin test. The modified Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing. Data were analyzed using SPSS version 26. Logistic regression analysis was used to determine the factors associated with vertical transmission of GBS, and statistical significance was set at <i>p</i> values <0.05.</p><p><strong>Result: </strong>The overall vertical transmission rate was 41.4% (67/162). History of preterm labor (Adjusted odds ratio (AOR) = 2.25; 95% CI: 1.11, 4.59), history of urinary tract infection (UTI) at current pregnancy (AOR = 2.25; 95% CI: 1.11, 4.59), and prolonged rupture of membranes greater than 18 hours (AOR = 2.23; 95% CI: 1.13, 4.4) were significantly associated with vertical transmission of GBS from previously colonized mothers to their newborn babies. Regarding GBS antibiotic susceptibility profile, a significant degree of resistance was observed to penicillin (29.9%), tetracycline (22.4%), ampicillin (20.9%), and clindamycin (19.4%).</p><p><strong>Conclusion: </strong>Our study documented a high prevalence of vertical transmission rate of GBS from pregnant women to their babies, with an overall transmission rate of 41.4%. The study identified the presence of antimicrobial-resistant GBS to penicillin, ampicillin, clindamycin, ciprofloxacin, and chloramphenicol. The organism was susceptible to vancomycin, followed by azithromycin, ceftriaxone, and erythromycin. Our study also reported MDR at 13.4%. Based on our findings, there is a need for antenatal culture-based GBS screening, maternal vaccination, and large-scale epidemiological and serotype identification studies to be put into practice in the study area.</p>\",\"PeriodicalId\":14098,\"journal\":{\"name\":\"International Journal of Microbiology\",\"volume\":\"2024 \",\"pages\":\"5673366\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-08-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316908/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/5673366\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/5673366","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:B 组链球菌(GBS)在妇女的直肠阴道部位定植,并垂直传播给新生儿。这种细菌与严重的新生儿并发症有关,包括肺炎、败血症和脑膜炎。GBS 对常用抗生素的耐药性增强。因此,我们对吉吉加市部分医院分娩的新生儿中 GBS 的垂直传播、诱因和抗菌药耐药性模式进行了评估。两家医院共对 849 名入住产房的孕妇进行了 GBS 定植筛查。随后,纳入了 162 名 GBS 定植孕妇及其新生儿。研究人员通过半结构式问卷调查和查阅病历,收集了研究对象的社会人口学和临床特征。经过培训的护士从孕妇的阴道和直肠部位以及新生儿的鼻腔、耳道和脐部采集拭子样本。将样本接种到添加了庆大霉素和萘啶酸的托德-休伊特肉汤培养基上,然后在血琼脂上进行亚培养。利用菌落特征、革兰氏染色和过氧化氢酶试验进行鉴定。所有革兰氏阳性球菌、乙型溶血性球菌和过氧化氢酶阴性球菌的分离物都通过克里斯蒂-阿特金斯-蒙克-彼得森(Christie-Atkins-Munch-Petersen)和杆菌肽试验进行了进一步鉴定。抗菌药敏感性测试采用改良柯比-鲍尔盘扩散法。数据使用 SPSS 26 版进行分析。逻辑回归分析用于确定与 GBS 垂直传播相关的因素,统计显著性以 p 值结果为标准:总垂直传播率为 41.4%(67/162)。早产史(调整后比值比 (AOR) = 2.25;95% CI:1.11, 4.59)、本次妊娠尿路感染(UTI)史(AOR = 2.25;95% CI:1.11, 4.59)和胎膜破裂时间超过 18 小时(AOR = 2.23;95% CI:1.13, 4.4)与 GBS 从以前有菌落的母亲垂直传播给新生儿有显著相关性。关于 GBS 的抗生素敏感性,观察到对青霉素(29.9%)、四环素(22.4%)、氨苄西林(20.9%)和林可霉素(19.4%)有明显的耐药性:我们的研究记录了孕妇将 GBS 垂直传播给婴儿的高流行率,总体传播率为 41.4%。研究发现了对青霉素、氨苄西林、克林霉素、环丙沙星和氯霉素耐药的 GBS。该病菌对万古霉素敏感,其次是阿奇霉素、头孢曲松和红霉素。我们的研究还报告了 13.4% 的 MDR。根据我们的研究结果,有必要在研究地区开展基于产前培养的 GBS 筛查、孕产妇疫苗接种以及大规模流行病学和血清型鉴定研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vertical Transmission of Group B Streptococcus, Prevalence, Associated Factors, and Antimicrobial Susceptibility Profile among Newborns Delivered at Health Facilities in Jigjiga City, Ethiopia.

Background: Group B Streptococcus (GBS) colonizes the rectovaginal area of women and vertically transmitted to neonates. This bacterium has been linked to severe neonatal complications including pneumonia, septicemia, and meningitis. GBS shows an increased resistance to commonly used antibiotics. Thus, we assessed the vertical transmission, contributing factors, and antimicrobial resistance patterns of GBS among newborns delivered at selected Hospitals in Jigjiga City.

Methods: Hospital-based cross-sectional study was conducted from 1st June 2022 to 30th April 2023. A total of 849 pregnant women admitted to delivery wards from two hospitals were screened for GBS colonization. Subsequently, 162 GBS-colonized pregnant women and their newborn babies were included. A semistructured questionnaire and a review of medical records were used to collect the sociodemographic and clinical characteristics of the study participants. Trained nurses collected swab samples from the vaginal-rectal area of pregnant women and the nasal, ear canal, and umbilical areas of newborn babies. Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. All gram-positive cocci, B-hemolytic, and catalase-negative isolates were further identified using Christie-Atkins-Munch-Petersen and a bacitracin test. The modified Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing. Data were analyzed using SPSS version 26. Logistic regression analysis was used to determine the factors associated with vertical transmission of GBS, and statistical significance was set at p values <0.05.

Result: The overall vertical transmission rate was 41.4% (67/162). History of preterm labor (Adjusted odds ratio (AOR) = 2.25; 95% CI: 1.11, 4.59), history of urinary tract infection (UTI) at current pregnancy (AOR = 2.25; 95% CI: 1.11, 4.59), and prolonged rupture of membranes greater than 18 hours (AOR = 2.23; 95% CI: 1.13, 4.4) were significantly associated with vertical transmission of GBS from previously colonized mothers to their newborn babies. Regarding GBS antibiotic susceptibility profile, a significant degree of resistance was observed to penicillin (29.9%), tetracycline (22.4%), ampicillin (20.9%), and clindamycin (19.4%).

Conclusion: Our study documented a high prevalence of vertical transmission rate of GBS from pregnant women to their babies, with an overall transmission rate of 41.4%. The study identified the presence of antimicrobial-resistant GBS to penicillin, ampicillin, clindamycin, ciprofloxacin, and chloramphenicol. The organism was susceptible to vancomycin, followed by azithromycin, ceftriaxone, and erythromycin. Our study also reported MDR at 13.4%. Based on our findings, there is a need for antenatal culture-based GBS screening, maternal vaccination, and large-scale epidemiological and serotype identification studies to be put into practice in the study area.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信