果蝇蛋白 Z4 具有 ZAD 二聚化结构域

Q3 Agricultural and Biological Sciences

Doklady Biological Sciences Pub Date : 2024-10-01 Epub Date: 2024-08-11 DOI:10.1134/S0012496624600179

A N Bonchuk, P G Georgiev

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引用次数: 0

摘要

转录因子 Z4（putzig）是决定果蝇染色质结构的关键蛋白之一。Z4 存在于多腺染色体上的带状边界，目前认为这些带状边界与染色质结构域相关。Z4 是一个蛋白质复合体的组成部分，该复合体还包括 Chromator 和 BEAF-32，Z4 的 N 端必须有一个保守结构域，以确保其与这两种蛋白质的相互作用。本研究在 Z4 中发现了一个锌指相关结构域（ZAD）。生化方法证实了该结构域的二聚能力。利用 AlphaFold2 获得了该结构域的二聚体模型，并利用小角 X 射线散射（SAXS）对模型结构进行了确认。二聚体结构显示了 ZAD 结构域的典型折叠。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Drosophila Protein Z4 Possesses ZAD Dimerization Domain.

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Drosophila Protein Z4 Possesses ZAD Dimerization Domain.

The transcription factor Z4 (putzig) is one of the key proteins that determine the chromatin structure in Drosophila. Z4 is found at the boundaries of bands on polytene chromosomes, and the bands are currently thought to correlate with chromatin domains. Z4 is a component of a protein complex that additionally includes Chromator and BEAF-32, and a conserved domain is necessary to occur at the N end of Z4 to ensure its interaction with the two proteins. In this study, a zinc finger-associated domain (ZAD) domain was identified in Z4. The capability of dimerization was confirmed for the domain by biochemical methods. A dimer model of the domain was obtained using AlphaFold2, and the model structure was confirmed using small-angle X-ray scattering (SAXS). The dimer structure shows a fold typical of ZAD domains.

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来源期刊

Doklady Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.