PCID2 M 域的点突变削弱了它在黑腹果蝇中输出 mRNA 的功能

Q3 Agricultural and Biological Sciences

Doklady Biological Sciences Pub Date : 2024-10-01 Epub Date: 2024-08-11 DOI:10.1134/S0012496624600167

Yu A Vdovina, S G Georgieva, D V Kopytova

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引用次数: 0

摘要

PCID2 蛋白是真核生物 TREX-2 复合物的一个组成部分，该复合物负责将 mRNA 从细胞核输出到细胞质中。我们先前已经证明，黑腹果蝇 PCID2 参与特异性 mRNA 识别，并确定了其与 ras2 RNA 相互作用的关键氨基酸。在这项工作中，氨基酸的点突变被证明会破坏 PCID2 与细胞 RNA 的相互作用，并扭曲果蝇细胞中含 polyA 的 mRNA 从细胞核输出到细胞质的过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Point Mutations in the M Domain of PCID2 Impair Its Function in mRNA Export in Drosophila melanogaster.

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Point Mutations in the M Domain of PCID2 Impair Its Function in mRNA Export in Drosophila melanogaster.

The PCID2 protein is a component of the eukaryotic TREX-2 complex, which is responsible for mRNA export from the nucleus into the cytoplasm. We have previously shown that Drosophila melanogaster PCID2 is involved in specific mRNA recognition and identified the key amino acids responsible for its interaction with the ras2 RNA. In this work, point mutations of the amino acids were shown to disrupt the PCID2 interaction with cell RNAs and to distort the export of polyA-containing mRNAs from the nucleus into the cytoplasm in Drosophila cells.

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来源期刊

Doklady Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.