A S Rahul Dev, S Harish, G Karthikeyan, M Nivedha, C Sangeetha
{"title":"链霉菌属联合体在番茄与花生芽坏死病毒相互作用过程中触发防御/PAMP 基因。","authors":"A S Rahul Dev, S Harish, G Karthikeyan, M Nivedha, C Sangeetha","doi":"10.1007/s13205-024-04030-6","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, <i>Streptomyces</i> spp. were isolated, characterized, and the efficacy was tested against <i>Groundnut bud necrosis orthotospovirus</i> (GBNV) in tomato. Among the three inoculation methods viz., pre-, post-, and simultaneous inoculation, tested for antiviral efficacy, pre-inoculation spray of the three <i>Streptomyces</i> spp. viz., <i>Streptomyces mutabilis, Streptomyces rochei,</i> and <i>Streptomyces chrestomyceticus</i> (SAT1, SAT4, and STR2) recorded the least disease severity index (DSI) of GBNV in tomato. In the pot culture, seed treatment of liquid consortium of three <i>Streptomyces</i> spp. @ 2 ml/g of seeds along with seedling dip at 10 ml/lit followed by soil drenching at 10 ml/lit on 7 days after transplanting (DAT) and foliar application at 0.5% on 15 DAT, 30 DAT, and 45 DAT recorded the least GBNV infection of 15% DSI and 16.67% DSI in trial I and II respectively. Besides, under field conditions, the disease incidence was reduced to 14.44% recording a higher yield of 76.67 t/ha in the treated plants against 63.99 t/ha in control. Upregulation of defense genes viz., PR1, PR2, PR6, WRKY, MAPKK, and NPR1 during tripartite interaction between tomato, <i>Streptomyces</i>, and GBNV was analyzed by qRTPCR, indicating that the consortia could decrease the virus severity through induced systemic resistance pathways. Thus, it is concluded that <i>Streptomyces</i> spp. can be used for the management of GBNV in tomato.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04030-6.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306705/pdf/","citationCount":"0","resultStr":"{\"title\":\"Consortia of <i>Streptomyces</i> spp. triggers defense/PAMP genes during the interaction of <i>Groundnut bud</i> <i>necrosis</i> <i>orthotospovirus</i> in tomato.\",\"authors\":\"A S Rahul Dev, S Harish, G Karthikeyan, M Nivedha, C Sangeetha\",\"doi\":\"10.1007/s13205-024-04030-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In the present study, <i>Streptomyces</i> spp. were isolated, characterized, and the efficacy was tested against <i>Groundnut bud necrosis orthotospovirus</i> (GBNV) in tomato. Among the three inoculation methods viz., pre-, post-, and simultaneous inoculation, tested for antiviral efficacy, pre-inoculation spray of the three <i>Streptomyces</i> spp. viz., <i>Streptomyces mutabilis, Streptomyces rochei,</i> and <i>Streptomyces chrestomyceticus</i> (SAT1, SAT4, and STR2) recorded the least disease severity index (DSI) of GBNV in tomato. In the pot culture, seed treatment of liquid consortium of three <i>Streptomyces</i> spp. @ 2 ml/g of seeds along with seedling dip at 10 ml/lit followed by soil drenching at 10 ml/lit on 7 days after transplanting (DAT) and foliar application at 0.5% on 15 DAT, 30 DAT, and 45 DAT recorded the least GBNV infection of 15% DSI and 16.67% DSI in trial I and II respectively. Besides, under field conditions, the disease incidence was reduced to 14.44% recording a higher yield of 76.67 t/ha in the treated plants against 63.99 t/ha in control. 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Consortia of Streptomyces spp. triggers defense/PAMP genes during the interaction of Groundnut budnecrosisorthotospovirus in tomato.
In the present study, Streptomyces spp. were isolated, characterized, and the efficacy was tested against Groundnut bud necrosis orthotospovirus (GBNV) in tomato. Among the three inoculation methods viz., pre-, post-, and simultaneous inoculation, tested for antiviral efficacy, pre-inoculation spray of the three Streptomyces spp. viz., Streptomyces mutabilis, Streptomyces rochei, and Streptomyces chrestomyceticus (SAT1, SAT4, and STR2) recorded the least disease severity index (DSI) of GBNV in tomato. In the pot culture, seed treatment of liquid consortium of three Streptomyces spp. @ 2 ml/g of seeds along with seedling dip at 10 ml/lit followed by soil drenching at 10 ml/lit on 7 days after transplanting (DAT) and foliar application at 0.5% on 15 DAT, 30 DAT, and 45 DAT recorded the least GBNV infection of 15% DSI and 16.67% DSI in trial I and II respectively. Besides, under field conditions, the disease incidence was reduced to 14.44% recording a higher yield of 76.67 t/ha in the treated plants against 63.99 t/ha in control. Upregulation of defense genes viz., PR1, PR2, PR6, WRKY, MAPKK, and NPR1 during tripartite interaction between tomato, Streptomyces, and GBNV was analyzed by qRTPCR, indicating that the consortia could decrease the virus severity through induced systemic resistance pathways. Thus, it is concluded that Streptomyces spp. can be used for the management of GBNV in tomato.
Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04030-6.
3 BiotechAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍:
3 Biotech publishes the results of the latest research related to the study and application of biotechnology to:
- Medicine and Biomedical Sciences
- Agriculture
- The Environment
The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.