急性低水平热中子的生物标记剂量测定及辐射对大鼠的适应性反应效应。

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Misara M Awad, Mahmoud H Abdelgawad, Eslam Aboelezz, Khairy T Ereiba
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引用次数: 0

摘要

在本文中,我们展示了急性低剂量中子对大鼠全身的生物效应,并研究了该剂量水平的中子对诱导体内放射适应反应的影响。为了解放射适应反应,受试动物分别接受了 5 毫希沃特和 10 毫希沃特的急性中子辐射剂量,14 天后又接受了 50 毫希沃特的挑战剂量。辐照后,所有接受单剂量和双剂量辐照的动物组在笼子里饲养一天后再取样。采用电子顺磁共振(EPR)法估算血液中辐射诱导的自由基,并测定一些血液学参数和脂质过氧化物(MDA)。对一些抗氧化酶[过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)]进行了彗星试验。根据中子暴露剂量对七组成年雄性大鼠进行了分类。结果表明,抗氧化酶的产量降低(CAT 降低 1.18-5.83%,SOD 降低 1.47-17.8%,GSH 降低 11.3-82.1%)。此外,在急性辐射剂量为 5 至 50 mSv 的所有组别中,红细胞分布宽度(RDW)(从 4.61% 增加到 25.19%)和彗星试验参数(如尾长(从 6.16 微米增加到 10.81 微米)、尾矩(从 1.17 微米增加到 2.46 微米)和尾长中 DNA 的百分比(DNA%)(从 9.58% 增加到 17.32%)分别有所增加。这表明,随着急性热中子剂量的增加,有害效应也在增加。研究中所有标记的无线电适应反应引入因子的值显示,较低的启动剂量会促进较高的适应反应,反之亦然。最终,研究结果表明,DNA%、SOD 酶水平、EPR 强度、总血红蛋白浓度和 RDWs 都有显著变化,这表明它们有可能被用作急性热中子剂量测定的生物标记。有必要开展进一步的研究,以验证这些测量结果作为辐照生物剂量学的有效性,包括研究不同挑战剂量和辐照后行为对 RAR 的响应影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biomarker dosimetry of acute low level of thermal neutrons and radiation adaptive response effect on rats.

Biomarker dosimetry of acute low level of thermal neutrons and radiation adaptive response effect on rats.

Biomarker dosimetry of acute low level of thermal neutrons and radiation adaptive response effect on rats.

Biomarker dosimetry of acute low level of thermal neutrons and radiation adaptive response effect on rats.

In this paper, we demonstrated the biological effects of acute low-dose neutrons on the whole body of rats and investigated the impact of that level of neutron dose to induce an in vivo radio-adaptive response. To understand the radio-adaptive response, the examined animals were exposed to acute neutron radiation doses of 5 and 10 mSv, followed by a 50 mSv challenge dose after 14 days. After irradiation, all groups receiving single and double doses were kept in cages for one day before sampling. The electron paramagnetic resonance (EPR) method was used to estimate the radiation-induced radicals in the blood, and some hematological parameters and lipid peroxidation (MDA) were determined. A comet assay was performed beside some of the antioxidant enzymes [catalase enzyme (CAT), superoxide dismutase (SOD), and glutathione (GSH)]. Seven groups of adult male rats were classified according to their dose of neutron exposure. Measurements of all studied markers are taken one week after harvesting, except for hematological markers, within 2 h. The results indicated lower production of antioxidant enzymes (CAT by 1.18-5.83%, SOD by 1.47-17.8%, and GSH by 11.3-82.1%). Additionally, there was an increase in red cell distribution width (RDW) (from 4.61 to 25.19%) and in comet assay parameters such as Tail Length, (from 6.16 to 10.81 µm), Tail Moment, (from 1.17 to 2.46 µm), and percentage of DNA in tail length (DNA%) (from 9.58 to 17.32%) in all groups exposed to acute doses of radiation ranging from 5 to 50 mSv, respectively. This emphasizes the ascending harmful effect with the increased acute thermal neutron doses. The values of the introduced factor of radio adaptive response for all markers under study reveal that the lower priming dose promotes a higher adaptation response and vice versa. Ultimately, the results indicate significant variations in DNA%, SOD enzyme levels, EPR intensity, total Hb concentration, and RDWs, suggesting their potential use as biomarkers for acute thermal neutron dosimetry. Further research is necessary to validate these measurements as biodosimetry for radiation exposure, including investigations involving the response impact of RAR with varied challenge doses and post-irradiation behavior.

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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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