Samuel Martin, Jose L Peiro, Marc Oria, Braxton Forde
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引用次数: 0
摘要
目的:与合成羊水(Amnio-well,AW)相比,正常生理盐水(NS)和乳酸林格氏液(LR)在体外会损伤人类羊膜上皮。我们试图评估在体内使用 NS、LR 和 AW 进行羊水交换的效果:在 E17.5 天,妊娠大鼠分别接受了 NS、LR 或 AW 羊水置换。每只妊娠大鼠中未进行羊膜腔穿刺的胎儿作为对照。在 E20.5 期收获羊水,并通过电子显微镜评估超微结构。通过 Western Blot 评估裂解基质金属蛋白酶 9(MMP9)和胶原 1(Col1a)的蛋白水平。通过免疫荧光(IF)评估连接蛋白-43的表达:结果:与对照组和 AW 相比,NS 和 LR 会导致羊膜微裂和上皮细胞萎缩。NS的MMP9/Col1裂解比率增加了3.9倍(p 结论:NS和LR的羊膜微裂和上皮细胞收缩比对照组和AW增加:与合成羊水相比,用 NS 和 LR 进行羊膜腔置换会导致羊膜微裂和胶原降解增加。需要更大的模型来验证或反驳这些发现。
Comparison of Amnio-Exchange With a Novel Synthetic Amniotic Fluid Versus Commercially Used Fluids for Fetal Therapy: An In Vivo Rodent Model.
Objective: Normal Saline (NS) and Lactated Ringer's (LR) damage human amniotic epithelium in vitro when compared with a synthetic amniotic fluid (Amnio-well, AW). We sought to evaluate the effect of amnio-exchange with NS, LR, and AW in vivo.
Methods: On day E17.5, pregnant rats underwent amnio-exchange with NS, LR, or AW. Fetuses in each pregnant rat that did not undergo amnio-exchange acted as controls. Amnions were harvested at E20.5 and ultrastructure evaluated via electron microscopy. Protein levels of cleaved matrix metalloproteinase 9 (MMP9) and collagen 1 (Col1a) were evaluated via Western Blot. Connexin-43 expression was evaluated via immunofluorescence (IF).
Results: There was an increase in amnion microfractures and epithelial cellular shrinkage with NS and LR compared with control and AW. The cleaved MMP9/Col1 ratio was increased 3.9-fold in NS (p < 0.001) and 4.5-fold LR (p = 0.0201) relative to control, whereas AW expression was similar to control (p = 0.636). Connexin-43 was also increased on IF in NS and LR relative to AW (mean gray intensity 26.5 ± 4.5, 26.5 ± 6.7, 19.2 ± 3.4, p < 0.001).
Conclusion: Amnio-exchange with NS and LR led to increased amniotic microfractures and collagen degradation compared with synthetic amniotic fluid. Larger models are warranted to validate or refute these findings.
期刊介绍:
Prenatal Diagnosis welcomes submissions in all aspects of prenatal diagnosis with a particular focus on areas in which molecular biology and genetics interface with prenatal care and therapy, encompassing: all aspects of fetal imaging, including sonography and magnetic resonance imaging; prenatal cytogenetics, including molecular studies and array CGH; prenatal screening studies; fetal cells and cell-free nucleic acids in maternal blood and other fluids; preimplantation genetic diagnosis (PGD); prenatal diagnosis of single gene disorders, including metabolic disorders; fetal therapy; fetal and placental development and pathology; development and evaluation of laboratory services for prenatal diagnosis; psychosocial, legal, ethical and economic aspects of prenatal diagnosis; prenatal genetic counseling