作为辣根过氧化物酶 (HRP) 金属酶简化类似物的星状多肽。

IF 4.4 4区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Macromolecular bioscience Pub Date : 2024-08-09 DOI:10.1002/mabi.202400155

Antoine Tronnet, Pedro Salas-Ambrosio, Rosa Roman, Lourdes Monica Bravo-Anaya, Marcela Ayala, Colin Bonduelle

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引用次数: 0

摘要

过氧化物酶，如辣根过氧化物酶（HRP），是一种血红素金属酶，是各种氧化反应的强大生物催化剂。通过使用简单的接枝-从方法、开环聚合（ROP）和锰卟啉，成功制备了能够催化 H2O2 氧化反应的 HRP 星形多肽类似物。与它们的蛋白质模型一样，这些简化的类似物在氧化剂的毫摩尔范围内显示出有趣的迈克尔斯-门顿常数（KM）。有趣的是，这些聚合物结构比 HRP 更耐变性（热、蛋白水解和氧化剂浓度），这为它们在催化或生物传感设备中的应用开辟了有趣的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Star-Like Polypeptides as Simplified Analogues of Horseradish Peroxidase (HRP) Metalloenzymes

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Star-Like Polypeptides as Simplified Analogues of Horseradish Peroxidase (HRP) Metalloenzymes

Peroxidases, like horseradish peroxidase (HRP), are heme metalloenzymes that are powerful biocatalysts for various oxidation reactions. By using simple grafting-from approach, ring-opening polymerization (ROP), and manganese porphyrins, star-shaped polypeptides analogues of HRP capable of catalyzing oxidation reactions with H₂O₂ is successfully prepared. Like their protein model, these simplified analogues show interesting Michaelis–Menten constant (K_M) in the mM range for the oxidant. Interestingly, the polymer structures are more resistant to denaturation (heat, proteolysis and oxidant concentration) than HRP, opening up interesting prospects for their use in catalysis or in biosensing devices.

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来源期刊

Macromolecular bioscience 生物-材料科学：生物材料

CiteScore

7.90

自引率

2.20%

发文量

211

审稿时长

1.5 months

期刊介绍： Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.