银纳米粒子对克隆尼生素对广泛耐药鲍曼不动杆菌抗菌活性的影响"。

IF 4.7 3区 医学 Q1 INFECTIOUS DISEASES
Meqdad Saleh Ahmed , Zirak.F.A. Abdulrahman , Zanan Mohammed Ameen Taha
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引用次数: 0

摘要

背景:抗生素耐药性是对人类健康的全球性威胁,会导致灾难。现有的抗生素无法控制鲍曼不动杆菌,这已成为一项挑战。因此,需要新型抗菌剂来应对此类威胁。本项目旨在寻找一种新型抗菌剂来治疗这种具有多重耐药性的细菌:方法:从乳酸乳球菌中分离出 NisA 基因,并使用 Gibson 克隆组装法将其克隆到 pET-3a 质粒中。将克隆纯化的 Nisin 与纳米银颗粒连接。最后,对纯化的 Nisin、银纳米颗粒和 Nisin-银纳米颗粒共轭物对广泛耐药的鲍曼尼氏菌的抗菌活性进行了评估:结果:从克隆细菌中成功纯化出 Nisin,浓度为 416 µg/ml。电子显微镜分析了尼生素和银纳米颗粒的共轭情况。尼生素和纳米银颗粒对鲍曼尼氏菌的最小抑菌浓度分别为 104 µg/ml 和 125 µg/ml。尼生素-银纳米颗粒共轭物显示出强大的抗菌活性,MIC 为 125-52 µg/ml,其中银纳米颗粒提高了尼生素的抗菌活性,超过了其最佳浓度(104 µg/ml)。结论:开发新的抗菌剂对控制广泛耐药细菌很有必要。与单独使用相比,尼生素-银共轭物显示出更强的抗菌活性,为抗击多重耐药鲍曼不动杆菌带来了希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of silver nanoparticles on the antimicrobial activity of cloned nisin against extensively drug-resistant Acinetobacter baumannii”

Background

Antibiotic resistance is a global threat to human health that leads to disasters. Acinetobacter baumannii cannot be controlled by the existing antibiotics, and it became challenging. Therefore, novel antibacterial agents are required to combat such threats. The aim of this project is to find a novel antimicrobial agent to treat this multi-drug resistant bacterium.

Methods

The NisA gene was isolated from Lactococcus lactis spp. lactis and cloned into the pET-3a plasmid using Gibson cloning assembly. Purified Nisin from cloning was conjugated with silver nanoparticles. Finally, an assessment of antibacterial activity for each of the purified Nisin, Silver nanoparticles, and Nisin-Silver nanoparticles conjugate against the extensively drug-resistant A. baumannii was performed.

Results

Nisin was successfully purified from cloned bacteria, and the concentration was 416 µg/ml. The conjugation of nisin and silver nanoparticles was analyzed by electron microscopy. The minimum inhibitory concentration of Nisin and silver nanoparticles against A. baumannii were 104 µg/ml and 125 µg/ml, respectively. While Nisin-silver nanoparticle conjugates showed potent antimicrobial activity with MIC 125–52 µg/ml in which silver nanoparticles increased the antimicrobial activity of nisin beyond its optimum concentration (104 µg/ml). ‎

Conclusion

The development of new antibacterial agents is necessary to control extensively drug-resistant bacteria. Nisin-silver conjugates showed more potent antimicrobial activity than when applied separately and gave hope to combat the multi-drug resistant A. baumannii.

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来源期刊
Journal of Infection and Public Health
Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES
CiteScore
13.10
自引率
1.50%
发文量
203
审稿时长
96 days
期刊介绍: The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.
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