Echis ocellatus 毒液诱导的大鼠精子功能缺陷、促凋亡和雄性生殖器官炎症活动:山柰醇的拮抗作用。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Babafemi Siji Ajisebiola, Adesola Abigeal Toromade, Johnson Olaleye Oladele, Abdur-Rahman Kolawole Mustapha, Olukunle Silas Fagbenro, Akindele Oluwatosin Adeyi
{"title":"Echis ocellatus 毒液诱导的大鼠精子功能缺陷、促凋亡和雄性生殖器官炎症活动:山柰醇的拮抗作用。","authors":"Babafemi Siji Ajisebiola, Adesola Abigeal Toromade, Johnson Olaleye Oladele, Abdur-Rahman Kolawole Mustapha, Olukunle Silas Fagbenro, Akindele Oluwatosin Adeyi","doi":"10.1186/s40360-024-00776-0","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Echis ocellatus envenoming is potentially toxic initiating clinical damages on male reproductive system. Kaempferol is a therapeutic agent with neutralizing potentials on snake venom toxins. This study investigated the antagonistic effect of kaempferol on E. ocellatus venom (EoV)-induced reproductive toxicities.</p><p><strong>Methods: </strong>Fifty adult male rats were sorted at random into five groups of ten rats for this study. The control rats were allotted to group 1, while rats in groups 2-5 were injected with 0.22 mg/kg bw (LD<sub>50</sub>) of EoV intraperitoneally. Rats in group 2 were not treated while groups 3-5 rats were treated with serum antivenom (0.2 ml), and 4 and 8 mg/kg bw of kaempferol post envenoming, respectively.</p><p><strong>Results: </strong>EoV actuated reproductive toxicity, significantly decreased sperm parameters, and enhanced inflammatory, oxidative stress, and apoptotic biomarkers in reproductive organs of untreated envenomed rats. However, treatment with kaempferol alleviated the venom-induced reproductive disorders with a dose dependent effect. Kaempferol significantly increased the testicular weight, organo-somatic index, sperm parameters, and normalized the levels of serum luteinizing hormone, testosterone, and follicle stimulating hormone. Kaempferol ameliorated testicular and epididymal oxidative stress as evidenced by significant decrease in malondialdehyde (MDA) levels, enhancement of reduced glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The inflammatory biomarkers; nitric oxide (NO) levels and myeloperoxidase activity (MPO), and apoptotic biomarkers; caspase 3 and caspase 9 activities were substantially suppressed in the testis and epididymis of envenomed rats treated with kaempferol.</p><p><strong>Conclusion: </strong>Results revealed kaempferol as a potential remedial agent against reproductive toxicity that could manifest post-viper envenoming.</p>","PeriodicalId":9023,"journal":{"name":"BMC Pharmacology & Toxicology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311923/pdf/","citationCount":"0","resultStr":"{\"title\":\"Echis ocellatus venom-induced sperm functional deficits, pro-apoptotic and inflammatory activities in male reproductive organs in rats: antagonistic role of kaempferol.\",\"authors\":\"Babafemi Siji Ajisebiola, Adesola Abigeal Toromade, Johnson Olaleye Oladele, Abdur-Rahman Kolawole Mustapha, Olukunle Silas Fagbenro, Akindele Oluwatosin Adeyi\",\"doi\":\"10.1186/s40360-024-00776-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Echis ocellatus envenoming is potentially toxic initiating clinical damages on male reproductive system. Kaempferol is a therapeutic agent with neutralizing potentials on snake venom toxins. This study investigated the antagonistic effect of kaempferol on E. ocellatus venom (EoV)-induced reproductive toxicities.</p><p><strong>Methods: </strong>Fifty adult male rats were sorted at random into five groups of ten rats for this study. The control rats were allotted to group 1, while rats in groups 2-5 were injected with 0.22 mg/kg bw (LD<sub>50</sub>) of EoV intraperitoneally. Rats in group 2 were not treated while groups 3-5 rats were treated with serum antivenom (0.2 ml), and 4 and 8 mg/kg bw of kaempferol post envenoming, respectively.</p><p><strong>Results: </strong>EoV actuated reproductive toxicity, significantly decreased sperm parameters, and enhanced inflammatory, oxidative stress, and apoptotic biomarkers in reproductive organs of untreated envenomed rats. However, treatment with kaempferol alleviated the venom-induced reproductive disorders with a dose dependent effect. Kaempferol significantly increased the testicular weight, organo-somatic index, sperm parameters, and normalized the levels of serum luteinizing hormone, testosterone, and follicle stimulating hormone. Kaempferol ameliorated testicular and epididymal oxidative stress as evidenced by significant decrease in malondialdehyde (MDA) levels, enhancement of reduced glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The inflammatory biomarkers; nitric oxide (NO) levels and myeloperoxidase activity (MPO), and apoptotic biomarkers; caspase 3 and caspase 9 activities were substantially suppressed in the testis and epididymis of envenomed rats treated with kaempferol.</p><p><strong>Conclusion: </strong>Results revealed kaempferol as a potential remedial agent against reproductive toxicity that could manifest post-viper envenoming.</p>\",\"PeriodicalId\":9023,\"journal\":{\"name\":\"BMC Pharmacology & Toxicology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-08-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311923/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Pharmacology & Toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s40360-024-00776-0\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Pharmacology & Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s40360-024-00776-0","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0

摘要

背景:蛇毒会对男性生殖系统造成临床损害。山奈酚是一种具有中和蛇毒潜力的治疗剂。本研究探讨了山奈酚对蛇毒(EoV)引起的生殖毒性的拮抗作用:本研究将 50 只成年雄性大鼠随机分为 5 组,每组 10 只。第 1 组为对照组,第 2-5 组大鼠腹腔注射 0.22 毫克/千克体重(半数致死剂量)的 EoV。第 2 组大鼠未接受治疗,而第 3-5 组大鼠则在毒液注射后分别接受血清抗蛇毒血清(0.2 毫升)和 4 毫克/千克体重和 8 毫克/千克体重的山柰酚治疗:结果:EoV对未经处理的大鼠造成生殖毒性,显著降低精子参数,并增强生殖器官的炎症、氧化应激和凋亡生物标志物。然而,使用山奈酚治疗可缓解毒液引起的生殖障碍,其效果与剂量有关。山奈酚能明显增加大鼠的睾丸重量、器官功能指数和精子参数,并使血清黄体生成素、睾酮和促卵泡激素的水平恢复正常。山奈酚改善了睾丸和附睾的氧化应激,表现在丙二醛(MDA)水平显著降低,还原型谷胱甘肽(GSH)水平、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性提高。使用山奈酚治疗的大鼠睾丸和附睾中的炎症生物标志物、一氧化氮(NO)水平和髓过氧化物酶活性(MPO),以及凋亡生物标志物、caspase 3 和 caspase 9 活性均受到大幅抑制:结论:研究结果表明,山奈酚是一种潜在的补救药物,可预防蝰蛇螫伤后可能出现的生殖毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Echis ocellatus venom-induced sperm functional deficits, pro-apoptotic and inflammatory activities in male reproductive organs in rats: antagonistic role of kaempferol.

Background: Echis ocellatus envenoming is potentially toxic initiating clinical damages on male reproductive system. Kaempferol is a therapeutic agent with neutralizing potentials on snake venom toxins. This study investigated the antagonistic effect of kaempferol on E. ocellatus venom (EoV)-induced reproductive toxicities.

Methods: Fifty adult male rats were sorted at random into five groups of ten rats for this study. The control rats were allotted to group 1, while rats in groups 2-5 were injected with 0.22 mg/kg bw (LD50) of EoV intraperitoneally. Rats in group 2 were not treated while groups 3-5 rats were treated with serum antivenom (0.2 ml), and 4 and 8 mg/kg bw of kaempferol post envenoming, respectively.

Results: EoV actuated reproductive toxicity, significantly decreased sperm parameters, and enhanced inflammatory, oxidative stress, and apoptotic biomarkers in reproductive organs of untreated envenomed rats. However, treatment with kaempferol alleviated the venom-induced reproductive disorders with a dose dependent effect. Kaempferol significantly increased the testicular weight, organo-somatic index, sperm parameters, and normalized the levels of serum luteinizing hormone, testosterone, and follicle stimulating hormone. Kaempferol ameliorated testicular and epididymal oxidative stress as evidenced by significant decrease in malondialdehyde (MDA) levels, enhancement of reduced glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The inflammatory biomarkers; nitric oxide (NO) levels and myeloperoxidase activity (MPO), and apoptotic biomarkers; caspase 3 and caspase 9 activities were substantially suppressed in the testis and epididymis of envenomed rats treated with kaempferol.

Conclusion: Results revealed kaempferol as a potential remedial agent against reproductive toxicity that could manifest post-viper envenoming.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信