PvMYB60 基因是在气候变化背景下提高普通豆类耐旱性的候选基因。

IF 4.3 2区 生物学 Q1 BIOLOGY
Vera Martínez-Barradas, Massimo Galbiati, Francisco Barco-Rubio, Dario Paolo, Carmen Espinoza, Eleonora Cominelli, Patricio Arce-Johnson
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引用次数: 0

摘要

背景:蚕豆(Phaseolus vulgaris)是世界上主要的营养资源之一,也是对环境影响较小的蛋白质来源。然而,其大部分种植区都受到干旱的影响,而且这种情况预计只会随着气候变化而恶化。气孔关闭是植物对干旱最重要的反应之一,而 MYB60 转录因子是调节气孔开度的关键因素之一。因此,我们研究了靶向突变蚕豆的 MYB60 基因是否是建立更耐旱蚕豆的一种有价值的策略:结果:研究发现,蚕豆的 MYB60 基因与拟南芥的 AtMYB60 基因具有同源关系,其保守区域具有 MYB60 基因的典型基序和结构。通过对含有气孔的器官和气孔丰富的叶片部分进行 q-RT PCR,进一步证实了 PvMYB60 的气孔特异性表达。此外,通过补充先前描述的拟南芥 myb60-1 突变体的缺陷表型,证实了 PvMYB60 在促进气孔开度方面的功能:我们的研究最终指出,在当前气候变化的背景下,PvMYB60 是获得更耐旱普通豆类的潜在靶标,这将进一步极大地促进粮食安全,尤其是易受干旱影响国家的粮食安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PvMYB60 gene, a candidate for drought tolerance improvement in common bean in a climate change context.

Background: Common bean (Phaseolus vulgaris) is one of the main nutritional resources in the world, and a low environmental impact source of protein. However, the majority of its cultivation areas are affected by drought and this scenario is only expected to worsen with climate change. Stomatal closure is one of the most important plant responses to drought and the MYB60 transcription factor is among the key elements regulating stomatal aperture. If targeting and mutating the MYB60 gene of common bean would be a valuable strategy to establish more drought-tolerant beans was therefore investigated.

Results: The MYB60 gene of common bean, with orthology to the Arabidopsis AtMYB60 gene, was found to have conserved regions with MYB60 typical motifs and architecture. Stomata-specific expression of PvMYB60 was further confirmed by q-RT PCR on organs containing stomata, and stomata-enriched leaf fractions. Further, function of PvMYB60 in promoting stomata aperture was confirmed by complementing the defective phenotype of a previously described Arabidopsis myb60-1 mutant.

Conclusions: Our study finally points PvMYB60 as a potential target for obtaining more drought-tolerant common beans in the present context of climate change which would further greatly contribute to food security particularly in drought-prone countries.

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来源期刊
Biological Research
Biological Research 生物-生物学
CiteScore
10.10
自引率
0.00%
发文量
33
审稿时长
>12 weeks
期刊介绍: Biological Research is an open access, peer-reviewed journal that encompasses diverse fields of experimental biology, such as biochemistry, bioinformatics, biotechnology, cell biology, cancer, chemical biology, developmental biology, evolutionary biology, genetics, genomics, immunology, marine biology, microbiology, molecular biology, neuroscience, plant biology, physiology, stem cell research, structural biology and systems biology.
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