Xueli Yuan, Jing Kong, Ying Xie, Xinxin Liu, Wei Zhang, Tao Liu, Zhenyu Chu, Wanqin Jin
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引用次数: 0
摘要
卡那霉素(KAN)的滥用污染了农牧产品、饮用水等,对人类健康的威胁与日俱增。因此,灵敏地检测实际样品中的痕量卡那霉素残留对于监测农业污染、确保食品安全和诊断疾病至关重要。然而,传统的 KAN 检测技术依赖于笨重的仪器和复杂的操作,检测限并不理想。在此,我们通过构建介孔DNA-钴@碳纳米纤维(DNA-Co@C-NFs)作为识别器,开发了一种新型的无标记诱导传感器,实现了对KAN的超灵敏检测。Co@C-NFs具有扩展的π-共轭结构、突出的比表面积和丰富的孔隙,可通过π-π堆叠相互作用有效地负载aptamer链,作为KAN的捕获器和报告器。由于结合 KAN 后 DNA 构型的变化,该灵敏传感器具有超低的检测限和超宽的线性范围,以及良好的精度和选择性。通过使用真实的自来水、牛奶和人体血清样本,该传感器准确地报告了痕量 KAN 的含量。因此,这种方便快捷的自动感应技术有望用于农业、食品安全和临床诊断中其他抗生素残留的现场检测。
The abuse of kanamycin (KAN) poses an increasing threat to human health by contaminating agricultural and animal husbandry products, drinking water, and more. Therefore, the sensitive detection of trace KAN residues in real samples is crucial for monitoring agricultural pollution, ensuring food safety, and diagnosing diseases. However, traditional assay techniques for KAN rely on bulky instruments and complicated operations with unsatisfactory detection limits. Herein, we developed a novel label-free aptasensor to achieve ultrasensitive detection of KAN by constructing mesoporous DNA-cobalt@carbon nanofibers (DNA-Co@C-NFs) as the recognizer. Leveraging the extended π-conjugation structure, prominent surface area, and abundant pores, the Co@C-NFs can effectively load aptamer strands via π-π stacking interactions, serving as KAN capturer and reporter. Due to the change in DNA configuration upon binding KAN, this aptasensor presented an ultralow detection limit and ultra-wide linear range, along with favorable precision and selectivity. Using real tap water, milk, and human serum samples, the aptasensor accurately reported trace KAN levels. As a result, this convenient and rapid autosensing technique holds promise for onsite testing of other antibiotic residues in agriculture, food safety, and clinical diagnosis.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.