Gordonia hongkongensis EUFUS-Z928 提取物的光保护相关特性:一种可培养的稀有放线菌,与加勒比八瓣珊瑚Eunicea fusca有关。

IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES
Jeysson Sánchez-Suárez, Luisa Villamil, Ericsson Coy-Barrera, Luis Díaz
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引用次数: 0

摘要

目前防晒霜配方中的紫外线过滤剂会对人体健康产生负面影响,如干扰内分泌和过敏反应,也会对环境产生负面影响,包括生物蓄积性和珊瑚健康毒性。因此,有必要寻找可作为更安全、更环保的活性成分的替代化合物。这项研究成功地从章鱼Eunicea fusca中分离出放线菌,并评估了它们作为光保护化合物生产者的潜力。使用生物基化学制剂,特别是天然产品,一直是发现生物活性化合物的高效策略,尤其是在海洋无脊椎动物及其相关微生物群中。我们获得了 18 种细菌分离物,随后利用它们在 Zobell 海洋肉汤中进行为期 7 天的浸没培养,制备甲醇提取物原液。对所得提取物进行了 2,2-二苯基-1-苦基肼(DPPH)和 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除能力筛选,并通过总酚和类黄酮含量测定对其进行表征。经过筛选,Gordonia hongkongensis EUFUS-Z928 提取物表现出最佳的抗氧化性,即每克提取物的 DPPH 和 ABTS 自由基清除能力分别为 4.93 和 6.00 µmol Trolox,并被选作进一步的光保护相关分析。因此,这种萃取物的紫外线吸收能力为体外防晒系数的 46.33%,而体外防晒系数是以 30 微克/毫升的羟苯甲酯计算得出的,但在浓度高达 500 微克/毫升时,它对人类真皮成纤维细胞(HDFa 细胞系)没有表现出任何细胞毒性。这种提取物的液相色谱-质谱化学特性分析表明,其化合物具有与清除自由基和吸收紫外线(即与光保护相关的活性)有关的结构特征。这些发现凸显了与 E. fusca 相关的微生物群的潜力,并证实了利用其代谢物实现光保护相关目的的可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Photoprotection-related properties of a raw extract from Gordonia hongkongensis EUFUS-Z928: A culturable rare actinomycete associated with the Caribbean octocoral Eunicea fusca.

UV filters in current sunscreen formulations can have negative effects on human health, such as endocrine disruption and allergic reactions, as well as on the environment, including bioaccumulation and coral health toxicity. As a result, there is a need to find alternative compounds that serve as safer and more ecofriendly active ingredients. This study successfully isolated actinomycetes from the octocoral Eunicea fusca and assessed their potential as producers of photoprotective compounds. The use of bio-based chemical agents, particularly natural products, has been a highly effective strategy for discovering bioactive compounds, especially in marine invertebrates and their associated microbiota. Eighteen bacterial isolates were obtained and subsequently employed to prepare raw methanolic extracts from seven-day submerged cultures in Zobell marine broth. The resulting extracts were screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity and characterized by total phenolic and flavonoid content measurements. After screening, the Gordonia hongkongensis EUFUS-Z928-derived raw extract exhibited the best antioxidant profile, i.e. DPPH and ABTS radical scavenging of 4.93 and 6.00 µmol Trolox per gram of extract, respectively, and selected for further photoprotection-related analysis. Thus, this extract demonstrated a UV-absorbing capacity of 46.33% of the in vitro sun protection factor calculated for 30 µg/mL oxybenzone but did not exhibit any cytotoxicity on human dermal fibroblasts (HDFa cell line) at concentrations up to 500 µg/mL. The liquid chromatography-mass spectrometry chemical characterization of this extract showed compounds with structural features associated with free radical scavenging and UV absorption (i.e. photoprotection-related activities). These findings highlighted the potential of the microbiota associated with E. fusca and confirmed the feasibility of exploiting its metabolites for photoprotection-related purposes.

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来源期刊
Science Progress
Science Progress Multidisciplinary-Multidisciplinary
CiteScore
3.80
自引率
0.00%
发文量
119
期刊介绍: Science Progress has for over 100 years been a highly regarded review publication in science, technology and medicine. Its objective is to excite the readers'' interest in areas with which they may not be fully familiar but which could facilitate their interest, or even activity, in a cognate field.
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