尼泊尔一家医院分离出的耐多药鲍曼不动杆菌的分子流行病学。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
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引用次数: 0

摘要

目的:耐多药(MDR)鲍曼不动杆菌的出现已成为一个严重的世界性医学问题。本研究旨在阐明 MDR 鲍曼不动杆菌临床分离株的遗传学和流行病学特性:方法:2019 年 5 月至 2020 年 2 月期间,从尼泊尔一所大学医院的 66 名住院患者中获得了 66 例 MDR 鲍曼尼杆菌分离株。采用新一代测序技术对这些分离株进行了全基因组测序。根据单核苷酸多态性共轭物构建了系统发生树。进行了多焦点序列分型(MLST)和克隆复合体(CC)分析,并鉴定了耐药基因:结果:在 66 个分离株中,26 个携带有编码 NDM 型金属-β-内酰胺酶的基因,55 个携带有编码 16S rRNA 甲基转移酶 ArmA 的基因。所有分离物的gyrA和parC的喹诺酮耐药性决定区都发生了点突变。系统发育分析表明,55 个分离株携带 armA,26 个携带 blaNDM-1,14 个携带 blaPER-7。MLST 和 CC 分析显示,34 个分离株属于 CC2(ST2),10 个属于 CC1(9 个 ST1 和 1 个 ST623),8 个属于 CC149(ST149)。与我们之前在2012年对尼泊尔MDR鲍曼尼菌的研究相比,2012年至2020年间,CC2的分离率有所上升,而CC149的分离率有所下降:本研究表明,产生碳青霉烯酶和16S rRNA甲基转移酶、对碳青霉烯类和/或氨基糖苷类药物具有高度耐药性的MDR鲍曼尼菌正在尼泊尔的医疗机构中蔓延。几年来,MDR 鲍曼尼氏菌分离株的基因背景已转向国际克隆 2。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular epidemiology of multidrug-resistant Acinetobacter baumannii isolates from a hospital in Nepal

Objectives

The emergence of multidrug-resistant (MDR) Acinetobacter baumannii has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR A. baumannii clinical isolates.

Methods

A total of 66 MDR A. baumannii isolates were obtained from 66 inpatients between May 2019 and February 2020 in a university hospital in Nepal. Whole genomes of these isolates were sequenced using next-generation sequencing. Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence typing (MLST) and clonal complex (CC) analysis were conducted, and drug-resistance genes were identified.

Results

Of the 66 isolates, 26 harboured a gene encoding NDM-type metallo-β-lactamase, and 55 harboured a gene encoding the 16S rRNA methyltransferase, ArmA. All isolates had point mutations in the quinolone-resistance-determining regions of gyrA and parC. Phylogenetic analysis showed that 55 isolates harboured armA, 26 harboured blaNDM-1, and14 harboured blaPER-7. Multilocus sequence typing and CC analysis revealed that 34 isolates belonged to CC2 (ST2), 10 to CC1 (nine ST1 and one ST623), and eight to CC149 (ST149). Compared to our previous study on MDR A. baumannii in Nepal in 2012, the isolation rate of CC2 increased, whereas that of CC149 decreased between 2012 and 2020.

Conclusions

This study indicates that MDR A. baumannii producing carbapenemase and 16S rRNA methyltransferase, with high resistance to carbapenems and/or aminoglycosides, are spreading in medical settings in Nepal. The genetic backgrounds of MDR A. baumannii isolates have shifted to international clone 2 over several years.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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