金黄色葡萄球菌生理学和 Agr 法定量感应对低剪切力模型微重力的响应。

IF 2.7 3区 生物学 Q3 MICROBIOLOGY
Journal of Bacteriology Pub Date : 2024-09-19 Epub Date: 2024-08-09 DOI:10.1128/jb.00272-24
Matthew R Hauserman, Leia E Sullivan, Kimberly L James, Mariola J Ferraro, Kelly C Rice
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引用次数: 0

摘要

金黄色葡萄球菌通常是从从太空飞行返回的宇航员体内分离出来的。之前对低地球轨道培养的金黄色葡萄球菌的全局数据分析显示,与地面对照组相比,太空飞行样本中Agr法定量感应系统及其下游目标的表达量明显增加。在本研究中,旋转细胞培养系统(RCCS)被用来研究低剪切模型微重力(LSMMG)对金黄色葡萄球菌生理机能和Agr活性的影响。与正常重力对照培养物相比,金黄色葡萄球菌 LSMMG 培养物在与之前太空飞行实验相同的生长介质和温度下培养时,agr 表达减少,生长方式也发生了改变,正常重力对照培养物通常在高倍率旋转容器(HARV)底部铺设充氧膜。当金黄色葡萄球菌以倒置的重力控制方向(充氧膜位于 HARV 的顶部)生长时,观察到 Agr 活性相对于传统对照和 LSMMG 培养物都有所降低,这表明氧气的可用性可能会影响观察到的 Agr 活性差异。代谢物检测显示,LSMMG 和倒置对照培养物的乳酸排泄量增加,乙酸排泄量减少。对 LSMMG、对照组和倒置对照组 HARV 培养上清液进行的分泌组学分析证实了这些结果,倒置和 LSMMG 培养物中 Agr 调控的毒力因子丰度降低,而在低氧条件下表达的蛋白质丰度增加。总之,这些研究表明,HARV 加氧膜的方向会影响 RCCS 中金黄色葡萄球菌的生理学和 Agr 法定量感应,在使用这种地面微重力模型解释数据时应考虑这一变量。宇航员的健康和免疫功能在太空飞行中会发生重大改变。因此,阐明微重力对金黄色葡萄球菌生理学的影响对于评估其在人类长期太空居住期间的致病可能性至关重要。这些结果还凸显了在比较模拟微重力模型数据和实际太空飞行实验时排除潜在干扰因素的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Response of Staphylococcus aureus physiology and Agr quorum sensing to low-shear modeled microgravity.

Staphylococcus aureus is commonly isolated from astronauts returning from spaceflight. Previous analysis of omics data from S. aureus low Earth orbit cultures indicated significantly increased expression of the Agr quorum sensing system and its downstream targets in spaceflight samples compared to ground controls. In this current study, the rotary cell culture system (RCCS) was used to investigate the effect of low-shear modeled microgravity (LSMMG) on S. aureus physiology and Agr activity. When cultured in the same growth medium and temperature as the previous spaceflight experiment, S. aureus LSMMG cultures exhibited decreased agr expression and altered growth compared to normal gravity control cultures, which are typically oriented with oxygenation membrane on the bottom of the high aspect rotating vessel (HARV). When S. aureus was grown in an inverted gravity control orientation (oxygenation membrane on top of the HARV), reduced Agr activity was observed relative to both traditional control and LSMMG cultures, signifying that oxygen availability may affect the observed differences in Agr activity. Metabolite assays revealed increased lactate and decreased acetate excretion in both LSMMG and inverted control cultures. Secretomics analysis of LSMMG, control, and inverted control HARV culture supernatants corroborated these results, with inverted and LSMMG cultures exhibiting a decreased abundance of Agr-regulated virulence factors and an increased abundance of proteins expressed in low-oxygen conditions. Collectively, these studies suggest that the orientation of the HARV oxygenation membrane can affect S. aureus physiology and Agr quorum sensing in the RCCS, a variable that should be considered when interpreting data using this ground-based microgravity model.IMPORTANCES. aureus is commonly isolated from astronauts returning from spaceflight and from surfaces within human-inhabited closed environments such as spacecraft. Astronaut health and immune function are significantly altered in spaceflight. Therefore, elucidating the effects of microgravity on S. aureus physiology is critical for assessing its pathogenic potential during long-term human space habitation. These results also highlight the necessity of eliminating potential confounding factors when comparing simulated microgravity model data with actual spaceflight experiments.

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来源期刊
Journal of Bacteriology
Journal of Bacteriology 生物-微生物学
CiteScore
6.10
自引率
9.40%
发文量
324
审稿时长
1.3 months
期刊介绍: The Journal of Bacteriology (JB) publishes research articles that probe fundamental processes in bacteria, archaea and their viruses, and the molecular mechanisms by which they interact with each other and with their hosts and their environments.
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