通过血清代谢组学技术筛选顺铂诱发大鼠急性肾损伤的早期生物标志物

Jinru Yang, Mingkang Zhang, Fenglin Ran, Xueyan Gou, Yanrong Ma, Xinan Wu
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引用次数: 0

摘要

研究目的系统鉴定顺铂诱导大鼠急性肾损伤(AKI)的早期生物标志物:实验研究。研究地点和时间研究地点:中国甘肃兰州大学实验动物实验室和中国甘肃兰州大学第一医院药学部,时间:2022年7月至2023年10月:本研究通过给大鼠连续注射顺铂建立AKI模型,对照组剂量为每天1毫克/千克,其他四组剂量分别为2天、3天、4天和5天。随后,采集大鼠血浆样本进行代谢组学分析,以确定血浆中肌酐升高前的早期分化代谢物。此外,还开发了精确的 HPLC-MS/MS 方法,以验证生物标记物在其他 AKI 模型中的变化:结果:第 4 天和第 5 天观察到了时间依赖性肾皮质损伤的发生和肌酐(Cr)浓度的显著变化,这表明模型的构建是成功的。第 2 天有 66 种化合物发生了变化,第 3 天有 61 种化合物发生了变化。有 11 种化合物的预测重要性(VIP)大于 1.5,错误发现率(FDR)为结论:N-乙酰谷氨酰胺和柠檬醛酸可作为顺铂诱导的急性肾损伤的早期生物标志物:急性肾损伤 生物标志物 顺铂 代谢组学 LC-MS/MS 大鼠
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening for Early Biomarkers of Cisplatin-Induced Acute Kidney Injury in Rats Through Serum Metabolomics Technology.

Objective: To systematically identify early biomarkers of cisplatin-induced acute kidney injury (AKI) in rats.

Study design: An experimental study. Place and Duration of the Study: Experimental Animal Laboratory of Lanzhou University, Gansu, China, and the Department of Pharmacy, The First Hospital of Lanzhou University, Gansu, China, from July 2022 to October 2023.

Methodology: In this study, an AKI model was established by continuously injecting cisplatin into rats at a dose of 1 mg/kg once a day for control group and for 2, 3, 4, and 5 days to other four groups, respectively. Subsequently, rat plasma samples were collected for metabolomics analysis to identify early differentiated metabolites in the plasma prior to creatinine elevation. Furthermore, accurate HPLC-MS/MS methods were developed to validate the biomarker variation in other AKI models.

Results: The occurrence of time-dependent renal cortical injury and significant alterations of creatinine (Cr) concentration were observed on day-4 and 5, which demonstrated successful model construction. Sixty-six compounds changed on Day-2 while 61 compounds changed on Day-3. Eleven compounds with variable importance in projection (VIP) >1.5 and false discover rate (FDR) <0.2 were selected and identified by HPLC-MS/MS. Among these, N-acetylglutamine and citramalic acid changed earlier than serum creatinine (sCr) in the AKI model.

Conclusion: N-acetylglutamine and citramalic acid may serve as early biomarker of cisplatin-induced AKI.

Key words: Acute kidney injury, Biomarker, Cisplatin, Metabolomics, LC-MS/MS, Rats.

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