{"title":"水稻总调节因子14h(GF14h)对低温种子萌发的影响及其在育种中的应用。","authors":"Yusaku Sugimura, Kaori Oikawa, Yu Sugihara, Hiroe Utsushi, Eiko Kanzaki, Kazue Ito, Yumiko Ogasawara, Tomoaki Fujioka, Hiroki Takagi, Motoki Shimizu, Hiroyuki Shimono, Ryohei Terauchi, Akira Abe","doi":"10.1371/journal.pgen.1011369","DOIUrl":null,"url":null,"abstract":"<p><p>Direct seeding is employed to circumvent the labor-intensive process of rice (Oryza sativa) transplantation, but this approach requires varieties with vigorous low-temperature germination (LTG) when sown in cold climates. To investigate the genetic basis of LTG, we identified the quantitative trait locus (QTL) qLTG11 from rice variety Arroz da Terra, which shows rapid seed germination at lower temperatures, using QTL-seq. We delineated the candidate region to a 52-kb interval containing GENERAL REGULATORY FACTOR14h (GF14h) gene, which is expressed during seed germination. The Arroz da Terra GF14h allele encodes functional GF14h, whereas Japanese rice variety Hitomebore harbors a 4-bp deletion in the coding region. Knocking out functional GF14h in a near-isogenic line (NIL) carrying the Arroz da Terra allele decreased LTG, whereas overexpressing functional GF14h in Hitomebore increased LTG, indicating that GF14h is the causal gene behind qLTG11. Analysis of numerous Japanese rice accessions revealed that the functional GF14h allele was lost from popular varieties during modern breeding. We generated a NIL in the Hitomebore background carrying a 172-kb genomic fragment from Arroz da Terra including GF14h. The NIL showed superior LTG compared to Hitomebore, with otherwise comparable agronomic traits. 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引用次数: 0
摘要
直接播种可避免水稻(Oryza sativa)移栽这一劳动密集型过程,但这种方法要求在寒冷气候条件下播种的品种具有旺盛的低温萌发(LTG)能力。为了研究LTG的遗传基础,我们利用QTL-seq从水稻品种Arroz da Terra中鉴定出了数量性状位点(QTL)qLTG11,该位点在较低温度下显示出种子的快速萌发。我们将候选区域划定为一个 52-kb 的区间,其中包含在种子萌发过程中表达的 GF14h (GENERAL REGULATORY FACTOR14h)基因。Arroz da Terra GF14h 等位基因编码功能性 GF14h,而日本水稻品种 Hitomebore 的编码区存在 4-bp 缺失。在携带 Arroz da Terra 等位基因的近等基因系(NIL)中敲除功能性 GF14h 会降低 LTG,而在 Hitomebore 中过表达功能性 GF14h 会增加 LTG,这表明 GF14h 是 qLTG11 的致病基因。对大量日本水稻品种的分析表明,功能性 GF14h 等位基因在现代育种过程中从流行品种中消失了。我们在 Hitomebore 背景下产生了一个 NIL,该 NIL 携带了来自 Arroz da Terra(包括 GF14h)的 172-kb 基因组片段。与 Hitomebore 相比,该 NIL 在其他农艺性状方面具有可比性,并表现出更优越的低温生长性状。来自 Arroz da Terra 的功能性 GF14h 等位基因是寒冷地区直接播种的宝贵资源。
Impact of rice GENERAL REGULATORY FACTOR14h (GF14h) on low-temperature seed germination and its application to breeding.
Direct seeding is employed to circumvent the labor-intensive process of rice (Oryza sativa) transplantation, but this approach requires varieties with vigorous low-temperature germination (LTG) when sown in cold climates. To investigate the genetic basis of LTG, we identified the quantitative trait locus (QTL) qLTG11 from rice variety Arroz da Terra, which shows rapid seed germination at lower temperatures, using QTL-seq. We delineated the candidate region to a 52-kb interval containing GENERAL REGULATORY FACTOR14h (GF14h) gene, which is expressed during seed germination. The Arroz da Terra GF14h allele encodes functional GF14h, whereas Japanese rice variety Hitomebore harbors a 4-bp deletion in the coding region. Knocking out functional GF14h in a near-isogenic line (NIL) carrying the Arroz da Terra allele decreased LTG, whereas overexpressing functional GF14h in Hitomebore increased LTG, indicating that GF14h is the causal gene behind qLTG11. Analysis of numerous Japanese rice accessions revealed that the functional GF14h allele was lost from popular varieties during modern breeding. We generated a NIL in the Hitomebore background carrying a 172-kb genomic fragment from Arroz da Terra including GF14h. The NIL showed superior LTG compared to Hitomebore, with otherwise comparable agronomic traits. The functional GF14h allele from Arroz da Terra represents a valuable resource for direct seeding in cold regions.
期刊介绍:
PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill).
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