ATG2 自噬相关蛋白通过将大量磷脂转移到病毒复制细胞器中,在番茄矮花叶病毒复制过程中发挥了病毒载体的作用。

IF 3.1 3区 生物学 Q3 CELL BIOLOGY
Molecular Biology of the Cell Pub Date : 2024-10-01 Epub Date: 2024-08-07 DOI:10.1091/mbc.E24-05-0236
Yuanrong Kang, Judit Pogany, Peter D Nagy
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引用次数: 0

摘要

正链 RNA 病毒利用细胞膜的颠覆和膜的增殖来构建支持病毒复制的病毒复制细胞器(VRO)。膜状 VRO 的生物生成需要受感染细胞的脂质代谢和脂质转移发生重大变化。在这项研究中,我们发现番茄矮花叶病毒(TBSV)通过与 TBSV p33 复制蛋白相互作用,将具有大量脂质转移活性的 Atg2 自噬相关蛋白劫持到 VRO 中。在酵母中删除 Atg2 和在烟草中敲除 Atg2 可减少 TBSV 的复制。我们发现,TBSV对Atg2的颠覆对于用磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)和PI(3)P磷脂酰肌醇苷富集VRO膜非常重要。有趣的是,抑制自噬并不影响 Atg2 被有效招募到 VRO 中,而 Atg2 的过度表达会增强 TBSV 的复制,这表明 TBSV 对 Atg2 的颠覆与自噬无关。这些发现表明,Atg2脂质转移蛋白的促病毒功能在于VRO膜增殖。此外,我们还发现,具有膜脂质扰乱酶活性的 Atg2 相互作用伙伴 Atg9 也参与了古墓病毒的复制。总之,Atg2 桥型脂质转移蛋白的颠覆性作用为古墓病毒提供了一种新的机制,使其能够极大地扩展 VRO 膜,从而支持病毒的强力复制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proviral role of ATG2 autophagy related protein in tomato bushy stunt virus replication through bulk phospholipid transfer into the viral replication organelle.

Subversion of cellular membranes and membrane proliferation are used by positive-strand RNA viruses to build viral replication organelles (VROs) that support virus replication. The biogenesis of the membranous VROs requires major changes in lipid metabolism and lipid transfer in infected cells. In this work, we show that tomato bushy stunt virus (TBSV) hijacks Atg2 autophagy related protein with bulk lipid transfer activity into VROs via interaction with TBSV p33 replication protein. Deletion of Atg2 in yeast and knockdown of Atg2 in Nicotiana benthamiana resulted in decreased TBSV replication. We found that subversion of Atg2 by TBSV was important to enrich VRO membranes with phosphatidylethanolamine (PE), phosphatidylserine (PS) and PI(3)P phosphoinositide. Interestingly, inhibition of autophagy did not affect the efficient recruitment of Atg2 into VROs, and overexpression of Atg2 enhanced TBSV replication, indicating autophagy-independent subversion of Atg2 by TBSV. These findings suggest that the proviral function of Atg2 lipid transfer protein is in VRO membrane proliferation. In addition, we find that Atg2 interacting partner Atg9 with membrane lipid-scramblase activity is also coopted for tombusvirus replication. Altogether, the subversion of Atg2 bridge-type lipid transfer protein provides a new mechanism for tombusviruses to greatly expand VRO membranes to support robust viral replication.

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来源期刊
Molecular Biology of the Cell
Molecular Biology of the Cell 生物-细胞生物学
CiteScore
6.00
自引率
6.10%
发文量
402
审稿时长
2 months
期刊介绍: MBoC publishes research articles that present conceptual advances of broad interest and significance within all areas of cell, molecular, and developmental biology. We welcome manuscripts that describe advances with applications across topics including but not limited to: cell growth and division; nuclear and cytoskeletal processes; membrane trafficking and autophagy; organelle biology; quantitative cell biology; physical cell biology and mechanobiology; cell signaling; stem cell biology and development; cancer biology; cellular immunology and microbial pathogenesis; cellular neurobiology; prokaryotic cell biology; and cell biology of disease.
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