Epstein-Barr 病毒 (EBV) 与鼻咽癌的关系：北印度一家三级医院的经验。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2024-08-09 DOI:10.1016/j.ijmmb.2024.100699

Debasish Biswal , Megha Brijwal , Aashish Choudhary , Aanchal Kakkar , Raja Pramanik , Alok Thakar , Lalit Dar

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引用次数: 0

摘要

简介鼻咽癌（NPC）是由爱泼斯坦-巴氏病毒（EBV）引起的鼻咽上皮癌。鼻咽癌常见于华南、东南亚和印度东北部。本研究的目的和目标是确定EBV在临床疑似鼻咽癌患者福尔马林固定石蜡包埋（FFPE）组织切片中的流行率，将聚合酶链反应（PCR）结果与组织病理学结果相关联，并确定组织EBV DNA作为诊断生物标志物的效用。材料和方法：从2018年4月至2019年12月，从临床疑似鼻咽癌患者处收集了31份FFPE组织样本。通过检查苏木精和伊红染色的切片进行组织病理学诊断。通过 EBNA-1 PCR 检测是否存在 EBV。采用 EBV 潜伏膜蛋白 1 进行 IHC 检测：在 31 例临床疑似鼻咽癌病例中，15 例（48.4%）经组织病理学确诊为鼻咽癌。其中13例（86.6%）为非角化性未分化鼻咽癌，角化性鼻咽癌和非角化性分化鼻咽癌各1例。在临床疑似鼻咽癌病例中，35.5%（11/31）的病例EBV EBNA1 PCR呈阳性。在11例PCR阳性病例中，9例（81.8%）经组织病理学确诊为鼻咽癌。在 31 例临床疑似鼻咽癌病例中，23 例活检结果显示 IHC 呈阳性。其中 12 例（52.2%）异常细胞中的 LMP1 呈阳性。在 12 份 IHC 阳性样本中，有 10 例为鼻咽癌病例：结论：将 EBV DNA 作为临床疑似病例的鼻咽癌指标，灵敏度为 60%，特异性为 87.5%。在这项研究中，通过对 FFPE 组织切片进行 PCR 检测，可在 20% 的病例中证实 EBV LMP1 IHC 检测不到的 EBV 相关性，从而有助于提高鼻咽癌病例中 EBV 阳性的检测率。鼻咽癌的早期诊断将提高治愈率，从而降低发病率和死亡率。

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Association of Epstein-Barr virus (EBV) with nasopharyngeal carcinoma: Experience from a North Indian tertiary care hospital

Introduction

Nasopharyngeal carcinoma (NPC), arising from nasopharyngeal epithelium is caused by Epstein-Barr virus (EBV). It is common in South China, South East Asia and North East India. The aim and objectives of this study were to determine the prevalence of EBV in formalin-fixed paraffin-embedded (FFPE) tissue sections of clinically suspected NPC patients, correlate the results of polymerase chain reaction (PCR) with histopathology findings, and to determine the utility of tissue EBV DNA as a diagnostic bio-marker.

Materials and methods

31 FFPE tissue samples were collected from clinically suspected NPC patients from April 2018–December 2019. Histopathological diagnosis was done by examination of Hematoxylin and Eosin stained slides. Presence of EBV was detected by EBNA-1 PCR. IHC was performed using EBV Latent Membrane Protein 1.

Results

Of the 31 clinically suspected NPC cases, 15 (48.4 %) were histopathological confirmed NPC. Of these15, 13 (86.6 %) were non-keratinising undifferentiated NPC, and one each were keratinising NPC and non-keratinising differentiated NPC respectively.

EBV EBNA1 PCR was positive in 35.5 % (11/31) of clinically suspected NPC cases. Of the 11 PCR positive cases, 9 (81.8 %) were histopathological confirmed NPC.

Of the 31 clinically suspected NPC cases, IHC was indicated in 23 biopsies. Of which, 12 (52.2 %) were positive for LMP1 in the abnormal cells. Of the 12 IHC positive samples, 10 were NPC cases.

Conclusion

EBV DNA as an indicator towards NPC among clinically suspected cases had a sensitivity of 60 % and specificity of 87.5 %. In this study, addition of EBV DNA detection by PCR from FFPE tissue sections could confirm EBV association in 20 % of cases where it was not detected by EBV LMP1 IHC, thus helped in increasing the detection of EBV positivity in NPC cases. Early diagnosis of NPC will improve the cure rate and hence reduce the morbidity and mortality rates.

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.