正电荷肽(PCPs)的羟自由基清除和化学修复能力:脉冲辐射分解研究。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-08-08 DOI:10.1080/10715762.2024.2385342
Chaozhong Tian, Shinichi Yamashita, Atsushi Kimura, Yui Obata, Hao Yu, Mitsumasa Taguchi
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引用次数: 0

摘要

脉冲辐射分解用于研究基本辐射化学反应,这些反应对 DNA 的辐射防护至关重要。研究涉及两种带正电荷的多肽(PCPs):组氨酸-酪氨酸-组氨酸(His-Tyr-His)和赖氨酸-酪氨酸-赖氨酸(Lys-Tyr-Lys)以及构成它们的氨基酸。酪氨酸(Tyr)、组氨酸(His)、赖氨酸(Lys)、His-Tyr-His 和 Lys-Tyr-Lys 与 OH 自由基(-OH)的反应速率常数分别为(1.6 ± 0.3)×1010、(9.0 ± 0.9)×109、(1.4±0.3)×109、(1.8±0.1)×1010 和(1.0±0.1)×1010 M-1s-1,表明肽键的形成会影响氨基酸与 -OH 的反应。观察到的瞬态吸收光谱表明,PCPs 两端的 His 或 Lys 残基对位于中心的 Tyr 有屏蔽作用。以脱氧鸟苷单磷酸(dGMP)为 DNA 模型进行的化学修复能力测量表明,Tyr、His-Tyr-His 和 Lys-Tyr-Lys 与 dGMP 自由基的反应速率常数分别为 (2.2 ± 0.5) × 108、(2.3 ± 0.1) × 108 和 (3.3 ± 0.4) × 108 M-1s-1,这意味着正电荷的存在可能会增强化学修复过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hydroxyl radical scavenging and chemical repair capabilities of positively charged peptides (PCPs): a pulse radiolysis study.

Pulse radiolysis was employed to investigate fundamental radiation chemical reactions, which are essential in the radiation protection of DNA. Two positively charged peptides (PCPs), histidine-tyrosine-histidine (His-Tyr-His) and lysine-tyrosine-lysine (Lys-Tyr-Lys), as well as the amino acids that constitute them, were involved. The reaction rate constants for tyrosine (Tyr), histidine (His), lysine (Lys), His-Tyr-His, and Lys-Tyr-Lys with OH radicals (OH) were (1.6 ± 0.3) × 1010, (9.0 ± 0.9) × 109, (1.4 ± 0.3) × 109, (1.8 ± 0.1) × 1010, and (1.0 ± 0.1) × 1010 M-1s-1, respectively, indicating that formation of peptide bond can affect the reaction of amino acids with OH. Observed transient absorption spectra indicated a shielding effect of the His or Lys residues at both ends of the PCPs on the centrally located Tyr. The measurement of chemical repair capabilities using deoxyguanosine monophosphate (dGMP) as a model for DNA demonstrated that the reaction rate constants of Tyr, His-Tyr-His, and Lys-Tyr-Lys with dGMP radicals were (2.2 ± 0.5) × 108, (2.3 ± 0.1) × 108, and (3.3 ± 0.4) × 108 M-1s-1, respectively, implying that the presence of a positive charge may enhance the chemical repair process.

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来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
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