MdNAC18 的自然变异通过调控乙烯生物合成基因对苹果果实的收获期产生了重要的遗传影响。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Guo Wen, Bei Wu, Yi Wang, Ting Wu, Zhenhai Han, Xinzhong Zhang
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引用次数: 0

摘要

将苹果果实采收期(AFHD)的遗传控制分解为多个孟德尔因子是现代遗传学面临的一项重大挑战。在此,我们将苹果丰产期的一个数量性状基因座(QTL)精细地映射到了由QTL Z03.5/Z03.6和F03.2/F03.3重叠定义的区间内的NAC转录因子(TF)MdNAC18上。MdNAC18 的一个直接靶标是乙烯生物合成基因 MdACO1。MdNAC18 编码序列中的单核苷酸多态性(SNPs)SNP517 和 SNP958 可调节 MdNAC18 对 MdACO1 的激活。MdACO1 启动子中的 SNP1229 破坏了 MdNAC18 的结合位点,因此取消了 MdNAC18 的结合。SNP517 和 SNP958 还影响了 MdNAC18 对 TF 基因 MdARF5 的激活;MdARF5 激活乙烯生物合成基因 MdACS1。MdNAC18中的SNP517和SNP958、MdACO1中的SNP1229和SNP769(与InDel62相关)以及MdACS1中的InDel162构成了一个遗传变异网络。据估计,该网络对 AFHD 的遗传效应为 60.3 d,占训练群体表型变异的 52.6%。这些多态性的共同作用提高了基因组学辅助预测(GAP)模型对AFHD的准确性(r = 0.7125)。总之,我们的研究结果表明,MdNAC18的遗传变异通过调节乙烯的生物合成影响AFHD,并为苹果育种提供了一个优化的GAP模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Natural variations in MdNAC18 exert major genetic effect on apple fruit harvest date by regulating ethylene biosynthesis genes.

Dissecting the genetic control of apple fruit harvest date (AFHD) into multiple Mendelian factors poses a significant challenge in modern genetics. Here, a quantitative trait locus (QTL) for AFHD was fine-mapped to the NAC transcription factor (TF) MdNAC18 within the interval defined by the overlap of QTLs Z03.5/Z03.6 and F03.2/F03.3. One direct target of MdNAC18 is the ethylene biosynthesis gene MdACO1. The single nucleotide polymorphisms (SNPs) SNP517 and SNP958 in the MdNAC18 coding sequence modulated activation of MdACO1 by MdNAC18. SNP1229 in the MdACO1 promoter destroyed the MdNAC18 binding site and thus abolished MdNAC18 binding. SNP517 and SNP958 also affected MdNAC18 activation of the TF gene MdARF5; MdARF5 activates the ethylene biosynthesis gene MdACS1. SNP517 and SNP958 in MdNAC18, SNP1229 and SNP769 (linked to InDel62) in MdACO1, and InDel162 in MdACS1 constituted a genetic variation network. The genetic effect of this network on AFHD was estimated as 60.3 d, accounting for 52.6% of the phenotype variation of the training population. The joint effects of these polymorphisms increased the accuracy of a genomics-assisted prediction (GAP) model for AFHD (r = 0.7125). Together, our results suggest that genetic variation in MdNAC18 affects AFHD by modulating ethylene biosynthesis and provide an optimized GAP model for apple breeding.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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