{"title":"干旱胁迫下喷洒褪黑素的紫杉(Taxus baccata L.)植物中的紫杉醇生物合成途径基因的生化反应和动态变化","authors":"Farnoosh Shahmohammadi, Marzieh Ghanbari Jahromi, Mohsen Farhadpour, Sepideh Kalateh Jari, Ali Mohammadi Torkashvand","doi":"10.1007/s11104-024-06890-6","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Aims</h3><p>This study aimed to assess the impact of exogenous melatonin on the expression of key genes in the taxol biosynthesis pathway (<i>DBAT</i> and <i>TXS</i>) and on taxol accumulation in <i>Taxus baccata</i> L. plants under different drought stress conditions. The research sought to determine how melatonin could modulate biochemical pathways to enhance plant resilience and secondary metabolite synthesis under stress.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>The research was structured as a factorial experiment using a randomized complete design, with melatonin treatments at concentrations of 0, 100, 200, and 300 µM and drought stress levels corresponding to 100, 80, 60, and 40% field capacity. The primary endpoints analyzed were gene expression, taxol accumulation, plant growth parameters, and secondary metabolite production.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The administration of 100 µM melatonin under mild drought conditions (80% FC) significantly enhanced the expression of the <i>DBAT</i> and <i>TXS</i> genes and resulted in the highest taxol production (1.93 mg g-1). Higher concentrations of melatonin (200 µM) were most effective in improving plant physiological traits including shoot and root biomass, height, and total chlorophyll content. Enhanced synthesis of phenolic and flavonoid compounds was particularly evident under moderate drought stress (60% FC) with melatonin treatments, underscoring an improved antioxidant capability.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Melatonin significantly improves drought resilience and stimulates the biosynthesis of taxol in <i>Taxus baccata</i>. These findings support the potential of melatonin in agricultural applications to boost plant growth, enhance stress tolerance, and increase the production of economically important secondary metabolites.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biochemical responses and dynamics of the taxol biosynthesis pathway genes in Taxus baccata L. plants sprayed with melatonin under drought stress\",\"authors\":\"Farnoosh Shahmohammadi, Marzieh Ghanbari Jahromi, Mohsen Farhadpour, Sepideh Kalateh Jari, Ali Mohammadi Torkashvand\",\"doi\":\"10.1007/s11104-024-06890-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Aims</h3><p>This study aimed to assess the impact of exogenous melatonin on the expression of key genes in the taxol biosynthesis pathway (<i>DBAT</i> and <i>TXS</i>) and on taxol accumulation in <i>Taxus baccata</i> L. plants under different drought stress conditions. The research sought to determine how melatonin could modulate biochemical pathways to enhance plant resilience and secondary metabolite synthesis under stress.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>The research was structured as a factorial experiment using a randomized complete design, with melatonin treatments at concentrations of 0, 100, 200, and 300 µM and drought stress levels corresponding to 100, 80, 60, and 40% field capacity. The primary endpoints analyzed were gene expression, taxol accumulation, plant growth parameters, and secondary metabolite production.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>The administration of 100 µM melatonin under mild drought conditions (80% FC) significantly enhanced the expression of the <i>DBAT</i> and <i>TXS</i> genes and resulted in the highest taxol production (1.93 mg g-1). Higher concentrations of melatonin (200 µM) were most effective in improving plant physiological traits including shoot and root biomass, height, and total chlorophyll content. Enhanced synthesis of phenolic and flavonoid compounds was particularly evident under moderate drought stress (60% FC) with melatonin treatments, underscoring an improved antioxidant capability.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p>Melatonin significantly improves drought resilience and stimulates the biosynthesis of taxol in <i>Taxus baccata</i>. These findings support the potential of melatonin in agricultural applications to boost plant growth, enhance stress tolerance, and increase the production of economically important secondary metabolites.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-024-06890-6\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-06890-6","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Biochemical responses and dynamics of the taxol biosynthesis pathway genes in Taxus baccata L. plants sprayed with melatonin under drought stress
Aims
This study aimed to assess the impact of exogenous melatonin on the expression of key genes in the taxol biosynthesis pathway (DBAT and TXS) and on taxol accumulation in Taxus baccata L. plants under different drought stress conditions. The research sought to determine how melatonin could modulate biochemical pathways to enhance plant resilience and secondary metabolite synthesis under stress.
Methods
The research was structured as a factorial experiment using a randomized complete design, with melatonin treatments at concentrations of 0, 100, 200, and 300 µM and drought stress levels corresponding to 100, 80, 60, and 40% field capacity. The primary endpoints analyzed were gene expression, taxol accumulation, plant growth parameters, and secondary metabolite production.
Results
The administration of 100 µM melatonin under mild drought conditions (80% FC) significantly enhanced the expression of the DBAT and TXS genes and resulted in the highest taxol production (1.93 mg g-1). Higher concentrations of melatonin (200 µM) were most effective in improving plant physiological traits including shoot and root biomass, height, and total chlorophyll content. Enhanced synthesis of phenolic and flavonoid compounds was particularly evident under moderate drought stress (60% FC) with melatonin treatments, underscoring an improved antioxidant capability.
Conclusions
Melatonin significantly improves drought resilience and stimulates the biosynthesis of taxol in Taxus baccata. These findings support the potential of melatonin in agricultural applications to boost plant growth, enhance stress tolerance, and increase the production of economically important secondary metabolites.
期刊介绍:
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.