白藜芦醇和乌拉地尔联合治疗对大鼠实验性脑缺血再灌注损伤的神经保护作用

Acta cirurgica brasileira Pub Date : 2024-08-05 eCollection Date: 2024-01-01 DOI:10.1590/acb395329
Rıdvan Çetin, Sinan Bahadir, İbrahim Basar, Barış Aslanoglu, Burak Atlas, Seval Kaya, Barış Can Güzel, Yahya Turan
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引用次数: 0

摘要

目的:评估白藜芦醇、脲吡地尔以及联合用药对大脑中动脉闭塞(MCAO)诱导的大鼠缺血再灌注(IR)损伤模型的神经保护作用:方法:35 只大鼠分为 5 组,每组 7 只。IR、IR 白藜芦醇(IRr)、IR 乌拉地尔(IRu)、IR + 白藜芦醇和乌拉地尔组合(IRc)组大鼠暴露于 MCAO 诱导的脑缺血再灌注损伤模型。IRr 和 IRu 组大鼠分别接受 30 毫克/千克的白藜芦醇和 5 毫克/千克的呋哒地尔治疗。IRc 组大鼠接受两种药物的联合治疗。研究结束后,对脑组织进行氧化应激(丙二醛、谷胱甘肽和超氧化物歧化酶)、促凋亡Caspase-3、抗凋亡Bcl-2和促炎症肿瘤坏死因子-α细胞因子水平测定:MCAO模型成功复制了红外损伤,与对照组相比,红外组组织病理变化明显,组织氧化应激升高,凋亡和炎症蛋白表达上调(p < 0.001)。与 IR 组相比,IRr 组的所有参数都明显减轻(均 p <0.05)。在 IRu 组中,除 caspase-3 和 Bcl-2 外,其他参数与 IR 组相比也有明显差异(均 p <0.05)。与 IR 组相比,IRc 组在所有参数上的差异最大(均 p < 0.001)。与 IRr 组和 IRu 组相比,IRc 组的超氧化物歧化酶和 Bcl-2 水平较高,而 Caspase-3 水平较低(均 p < 0.05)。此外,与IRu组相比,IRc组的MDA和TNF-α水平较低(均为P < 0.05):结果表明,白藜芦醇和脲吡地尔的联合治疗可能是一种降低脑 IR 损伤中神经退行性变的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neuroprotective effects of the combined treatment of resveratrol and urapidil in experimental cerebral ischemia-reperfusion injury in rats.

Purpose: To evaluate the neuroprotective effect of resveratrol, urapidil, and a combined administration of these drugs against middle cerebral artery occlusion (MCAO) induced ischemia/reperfusion (IR) injury model in rats.

Methods: Thirty-five rats were divided into five groups of seven animals each. Animals in IR, IR resveratrol (IRr), IR urapidil (IRu), and IR + combination of resveratrol and urapidil (IRc) were exposed to MCAO induced cerebral ischemia reperfusion injury model. Rats in IRr and IRu groups received 30-mg/kg resveratrol and 5-mg/kg urapidil respectively. Animals in IRc received a combined treatment of both drugs. At the end of the study, brain tissues were used for oxidative stress (malondialdehyde, glutathione, and superoxide dismutase), pro-apoptotic caspase-3, anti-apoptotic Bcl-2, and pro-inflammatory tumor necrosis factor-α cytokine level measurements.

Results: The MCAO model successfully replicated IR injury with significant histopathological changes, elevated tissue oxidative stress, and upregulated apoptotic and inflammatory protein expression in IR group compared to control group (p < 0.001). All parameters were significantly alleviated in IRr group compared to IR group (all p < 0.05). In IRu group, all parameters except for caspase-3 and Bcl-2 were also significantly different than IR group (all p < 0.05). The IRc group showed the biggest difference compared to IR group in all parameters (all p < 0.001). The IRc had higher superoxide dismutase and Bcl-2 levels, and lower caspase-3 levels compared to both IRr and IRu groups (all p < 0.05). Also, the IRc group had lower MDA and TNF-α levels compared to IRu group (all p < 0.05).

Conclusions: The results indicate that combined treatment of resveratrol and urapidil may be a novel strategy to downregulate neurodegeneration in cerebral IR injury.

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