中国 26 个省份人工饲养蛇类中的隐孢子虫属:流行率、分子特征和症状。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI:10.1051/parasite/2024047
Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang
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引用次数: 0

摘要

蛇有时被视为宠物,并被用于传统中药。蛇体内经常发现隐孢子虫属,是一种重要的病原体,可引起胃肠道疾病。目前的数据表明,不同隐孢子虫属的感染风险因素和临床症状表现模式可能不同。 为了更好地了解隐孢子虫属的感染状况,我们在中国 26 个省份收集了 603 条无症状蛇和 147 条有症状蛇的粪便样本。这些样本分别来自蝮蛇(Elaphe guttata)、乌梢蛇(Elaphe obsoleta)、蝮蛇(Pituophis melanoleucus)、蝮蛇(Thamnophis sirtalis)、蝮蛇(Lampropeltis getulus)和蝮蛇(Heterodon nasicus)。利用巢式聚合酶链反应(PCR)扩增了部分小亚基(SSU)rRNA基因,以调查隐孢子虫属的感染率,并评估其进化关系和遗传特征。无症状蛇的感染率为 20%,年龄是一个重要的风险因素。相比之下,70%的无症状蛇对隐孢子虫属呈阳性反应,其中包括蛇形隐孢子虫和变种隐孢子虫(C. saurophilum)。进一步的分析表明,蛇形隐孢子虫与反胃有潜在的联系,varanii 隐孢子虫与腹泻有潜在的联系,而这两种隐孢子虫都与胀气无关。据我们所知,这是首次报道中国有症状的蛇体内隐孢子虫属和相关临床症状的研究。本研究旨在加深对蛇类隐孢子虫感染、风险因素和临床表现的了解,为隐孢子虫病的控制和预防提供重要数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms.

Snakes are sometimes regarded as pets and are used in traditional Chinese medicine. Cryptosporidium spp. are frequently identified in snakes, representing an important pathogen and causing gastrointestinal diseases. Current data indicate that risk factors for infection and patterns of clinical symptom presentation may differ among Cryptosporidium spp. To better understand the infection status by Cryptosporidium spp., fecal samples were collected from 603 asymptomatic and 147 symptomatic snakes in 26 provinces of China. These samples came from Elaphe guttata, Elaphe obsoleta, Pituophis melanoleucus, Thamnophis sirtalis, Lampropeltis getulus, and Heterodon nasicus. The partial small subunit (SSU) rRNA gene was amplified using nested polymerase chain reaction (PCR) to investigate the infection rate of Cryptosporidium spp., and to assess evolutionary relationships and genetic characterization. A prevalence of 20% was recorded in asymptomatic snakes, with age identified as a significant risk factor. In contrast, 70% of symptomatic snakes were positive for Cryptosporidium spp., with Cryptosporidium serpentis and Cryptosporidium varanii (syn. C. saurophilum). Further analysis revealed a potential association between C. serpentis and regurgitation, and C. varanii and diarrhea, while neither species was linked to flatulence. To our knowledge, this is the first study to report Cryptosporidium spp. and associated clinical signs in symptomatic snakes in China. This study aims to enhance the understanding of Cryptosporidium infections, risk factors, and clinical manifestations in snakes, providing data crucial for the control and prevention of cryptosporidiosis.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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