Kyun-Hee Lee, Moonju Hong, Haeng Jeon Hur, Mi Jeong Sung, Ae Sin Lee, Min Jung Kim, Hye Jeong Yang, Myung-Sunny Kim
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引用次数: 0
摘要
简介：人参莓果（GB）曾被证实能改善饮食诱导肥胖（DIO）小鼠的全身胰岛素抵抗，调节肝糖代谢和脂肪变性：本研究采用代谢组学分析方法评估了GB在DIO小鼠肝脏总代谢物中的作用：方法：采用毛细管电泳飞行时间质谱法（CE-TOF/MS）对12周正常膳食（NC）、高脂膳食（HFD）和添加0.1% GB的高脂膳食（HFD + GB）小鼠的肝组织进行代谢组学分析。通过偏最小二乘判别分析（PLS-DA）、小分子通路数据库（SMPDB）和 MetaboAnalyst 5.0 对检测到的代谢物、其通路和功能进行了分析：NC、HFD和GB喂养小鼠（HFD + GB）的肝脏代谢物谱高度分区。涉及线粒体功能、糖代谢/糖酵解、脂肪酸代谢和初级胆汁酸生物合成等主要肝功能的代谢物在摄入 GB 后出现差异。与空腹血糖（FBG）、胰岛素和胰岛素抵抗平衡模型评估（HOMA-IR）呈显著相关的代谢物与线粒体膜功能、能量平衡和糖代谢高度相关。人参浆果的摄入增加了线粒体膜功能相关代谢物的水平，降低了葡萄糖代谢相关代谢物的水平，并与代谢表型高度相关：本研究表明，长期摄入人参皂苷改变了 DIO 小鼠肝衰竭肝脏的代谢物，使肝脏中涉及线粒体功能和糖代谢的代谢物趋于正常，并表明了人参皂苷改善 DIO 小鼠高血糖的潜在机制。
Metabolomic profiling analysis reveals the benefits of ginseng berry intake on mitochondrial function and glucose metabolism in the liver of obese mice.
Introduction: Ginseng berry (GB) has previously been demonstrated to improve systemic insulin resistance and regulate hepatic glucose metabolism and steatosis in mice with diet-induced obesity (DIO).
Objectives: In this study, the role of GB in metabolism was assessed using metabolomics analysis on the total liver metabolites of DIO mice.
Methods: Metabolomic profiling was performed using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF/MS) of liver tissue from mice on a 12-wk normal chow diet (NC), high-fat diet (HFD), and HFD supplemented with 0.1% GB (HFD + GB). The detected metabolites, its pathways, and functions were analyzed through partial least square discriminant analysis (PLS-DA), the small molecular pathway database (SMPDB), and MetaboAnalyst 5.0.
Results: The liver metabolite profiles of NC, HFD, and GB-fed mice (HFD + GB) were highly compartmentalized. Metabolites involved in major liver functions, such as mitochondrial function, gluconeogenesis/glycolysis, fatty acid metabolism, and primary bile acid biosynthesis, showed differences after GB intake. The metabolites that showed significant correlations with fasting blood glucose (FBG), insulin, and homeostatic model assessment for insulin resistance (HOMA-IR) were highly associated with mitochondrial membrane function, energy homeostasis, and glucose metabolism. Ginseng berry intake increased the levels of metabolites involved in mitochondrial membrane function, decreased the levels of metabolites related to glucose metabolism, and was highly correlated with metabolic phenotypes.
Conclusion: This study demonstrated that long-term intake of GB changed the metabolite of hepatosteatotic livers in DIO mice, normalizing global liver metabolites involved in mitochondrial function and glucose metabolism and indicating the potential mechanism of GB in ameliorating hyperglycemia in DIO mice.
期刊介绍：
Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to:
metabolomic applications within man, including pre-clinical and clinical
pharmacometabolomics for precision medicine
metabolic profiling and fingerprinting
metabolite target analysis
metabolomic applications within animals, plants and microbes
transcriptomics and proteomics in systems biology
Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.
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