{"title":"利用 Prompt 预测多种原核生物的启动子","authors":"Qimeng Du, Yixue Guo, Junpeng Zhang, Fuping Lu, Chong Peng, Chichun Zhou","doi":"10.1007/s12539-024-00637-8","DOIUrl":null,"url":null,"abstract":"<p><p>Promoters are important cis-regulatory elements for the regulation of gene expression, and their accurate predictions are crucial for elucidating the biological functions and potential mechanisms of genes. Many previous prokaryotic promoter prediction methods are encouraging in terms of the prediction performance, but most of them focus on the recognition of promoters in only one or a few bacterial species. Moreover, due to ignoring the promoter sequence motifs, the interpretability of predictions with existing methods is limited. In this work, we present a generalized method Prompt (Promoters in multiple prokaryotes) to predict promoters in 16 prokaryotes and improve the interpretability of prediction results. Prompt integrates three methods including RSK (Regression based on Selected k-mer), CL (Contrastive Learning) and MLP (Multilayer Perception), and employs a voting strategy to divide the datasets into high-confidence and low-confidence categories. Results on the promoter prediction tasks in 16 prokaryotes show that the accuracy (Accuracy, Matthews correlation coefficient) of Prompt is greater than 80% in highly credible datasets of 16 prokaryotes, and is greater than 90% in 12 prokaryotes, and Prompt performs the best compared with other existing methods. Moreover, by identifying promoter sequence motifs, Prompt can improve the interpretability of the predictions. Prompt is freely available at https://github.com/duqimeng/PromptPrompt , and will contribute to the research of promoters in prokaryote.</p>","PeriodicalId":13670,"journal":{"name":"Interdisciplinary Sciences: Computational Life Sciences","volume":" ","pages":"814-828"},"PeriodicalIF":3.9000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Predicting Promoters in Multiple Prokaryotes with Prompt.\",\"authors\":\"Qimeng Du, Yixue Guo, Junpeng Zhang, Fuping Lu, Chong Peng, Chichun Zhou\",\"doi\":\"10.1007/s12539-024-00637-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Promoters are important cis-regulatory elements for the regulation of gene expression, and their accurate predictions are crucial for elucidating the biological functions and potential mechanisms of genes. Many previous prokaryotic promoter prediction methods are encouraging in terms of the prediction performance, but most of them focus on the recognition of promoters in only one or a few bacterial species. Moreover, due to ignoring the promoter sequence motifs, the interpretability of predictions with existing methods is limited. In this work, we present a generalized method Prompt (Promoters in multiple prokaryotes) to predict promoters in 16 prokaryotes and improve the interpretability of prediction results. Prompt integrates three methods including RSK (Regression based on Selected k-mer), CL (Contrastive Learning) and MLP (Multilayer Perception), and employs a voting strategy to divide the datasets into high-confidence and low-confidence categories. Results on the promoter prediction tasks in 16 prokaryotes show that the accuracy (Accuracy, Matthews correlation coefficient) of Prompt is greater than 80% in highly credible datasets of 16 prokaryotes, and is greater than 90% in 12 prokaryotes, and Prompt performs the best compared with other existing methods. Moreover, by identifying promoter sequence motifs, Prompt can improve the interpretability of the predictions. Prompt is freely available at https://github.com/duqimeng/PromptPrompt , and will contribute to the research of promoters in prokaryote.</p>\",\"PeriodicalId\":13670,\"journal\":{\"name\":\"Interdisciplinary Sciences: Computational Life Sciences\",\"volume\":\" \",\"pages\":\"814-828\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Interdisciplinary Sciences: Computational Life Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12539-024-00637-8\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MATHEMATICAL & COMPUTATIONAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Interdisciplinary Sciences: Computational Life Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12539-024-00637-8","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MATHEMATICAL & COMPUTATIONAL BIOLOGY","Score":null,"Total":0}
Predicting Promoters in Multiple Prokaryotes with Prompt.
Promoters are important cis-regulatory elements for the regulation of gene expression, and their accurate predictions are crucial for elucidating the biological functions and potential mechanisms of genes. Many previous prokaryotic promoter prediction methods are encouraging in terms of the prediction performance, but most of them focus on the recognition of promoters in only one or a few bacterial species. Moreover, due to ignoring the promoter sequence motifs, the interpretability of predictions with existing methods is limited. In this work, we present a generalized method Prompt (Promoters in multiple prokaryotes) to predict promoters in 16 prokaryotes and improve the interpretability of prediction results. Prompt integrates three methods including RSK (Regression based on Selected k-mer), CL (Contrastive Learning) and MLP (Multilayer Perception), and employs a voting strategy to divide the datasets into high-confidence and low-confidence categories. Results on the promoter prediction tasks in 16 prokaryotes show that the accuracy (Accuracy, Matthews correlation coefficient) of Prompt is greater than 80% in highly credible datasets of 16 prokaryotes, and is greater than 90% in 12 prokaryotes, and Prompt performs the best compared with other existing methods. Moreover, by identifying promoter sequence motifs, Prompt can improve the interpretability of the predictions. Prompt is freely available at https://github.com/duqimeng/PromptPrompt , and will contribute to the research of promoters in prokaryote.
期刊介绍:
Interdisciplinary Sciences--Computational Life Sciences aims to cover the most recent and outstanding developments in interdisciplinary areas of sciences, especially focusing on computational life sciences, an area that is enjoying rapid development at the forefront of scientific research and technology.
The journal publishes original papers of significant general interest covering recent research and developments. Articles will be published rapidly by taking full advantage of internet technology for online submission and peer-reviewing of manuscripts, and then by publishing OnlineFirstTM through SpringerLink even before the issue is built or sent to the printer.
The editorial board consists of many leading scientists with international reputation, among others, Luc Montagnier (UNESCO, France), Dennis Salahub (University of Calgary, Canada), Weitao Yang (Duke University, USA). Prof. Dongqing Wei at the Shanghai Jiatong University is appointed as the editor-in-chief; he made important contributions in bioinformatics and computational physics and is best known for his ground-breaking works on the theory of ferroelectric liquids. With the help from a team of associate editors and the editorial board, an international journal with sound reputation shall be created.