Iqra Bashir, Muhammad Hidayat Rasool, Muhammad Shafique, Kokab Jabeen, Muhammad Usman Qamar
{"title":"探索麦卢卡蜂蜜对巴基斯坦引起败血症的耐多药和广泛耐药伤寒沙门氏菌的抗菌功效。","authors":"Iqra Bashir, Muhammad Hidayat Rasool, Muhammad Shafique, Kokab Jabeen, Muhammad Usman Qamar","doi":"10.1080/17460913.2024.2384260","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of <i>Salmonella</i> Typhi. <b>Materials & methods:</b> Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. <b>Results:</b> MDR and XDR-<i>S.</i> Typhi was susceptible to azithromycin. These strains carried the H58, <i>gyrA</i>, <i>gyrB</i>, <i>bla</i><sub>CTX-M-15</sub> , and <i>bla</i><sub>TEM-1</sub> genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. <b>Conclusion:</b> Manuka honey could be an alternative option for treating <i>S.</i> Typhi infections.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant <i>Salmonella</i> Typhi causing septicemia in Pakistan.\",\"authors\":\"Iqra Bashir, Muhammad Hidayat Rasool, Muhammad Shafique, Kokab Jabeen, Muhammad Usman Qamar\",\"doi\":\"10.1080/17460913.2024.2384260\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of <i>Salmonella</i> Typhi. <b>Materials & methods:</b> Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. <b>Results:</b> MDR and XDR-<i>S.</i> Typhi was susceptible to azithromycin. These strains carried the H58, <i>gyrA</i>, <i>gyrB</i>, <i>bla</i><sub>CTX-M-15</sub> , and <i>bla</i><sub>TEM-1</sub> genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. <b>Conclusion:</b> Manuka honey could be an alternative option for treating <i>S.</i> Typhi infections.</p>\",\"PeriodicalId\":12773,\"journal\":{\"name\":\"Future microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/17460913.2024.2384260\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17460913.2024.2384260","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Salmonella Typhi causing septicemia in Pakistan.
Aim: To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of Salmonella Typhi. Materials & methods: Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. Results: MDR and XDR-S. Typhi was susceptible to azithromycin. These strains carried the H58, gyrA, gyrB, blaCTX-M-15 , and blaTEM-1 genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. Conclusion: Manuka honey could be an alternative option for treating S. Typhi infections.
期刊介绍:
Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.