从节肢动物内脏(Methanolapillus gen.)

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Evgenii Protasov, Hanna Reeh, Pengfei Liu, Anja Poehlein, Katja Platt, Thomas Heimerl, Vincent Hervé, Rolf Daniel, Andreas Brune
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引用次数: 0

摘要

最近的元基因组研究发现了许多依赖氢气的强制性甲基还原甲烷菌系。然而,在纯培养物中分离出的代表物种却寥寥无几。在这里,我们描述了具有这种能力的 Methanosarcinaceae 科(Methanosarcinales 目)的六个新物种,它们在节肢动物内脏的甲烷菌群落中占了很大一部分。系统发生组分析将蟑螂后肠的分离物归入 Methanimicrococcus 属(M. hacksteinii、M. hongohii、M. stummii),将千足虫后肠的分离物归入一个新属 Methanolapillus(M. africanus、M. millepedarum、M. ohkumae)。该肠道支系的成员还包括来自白蚁和脊椎动物的未培养代表,其基因组(1.6-2.2 Mbp)比其他甲壳动物小得多。基因组的缩小伴随着伍德-荣格达尔途径上半部分、几个能量转换膜复合体(Fpo、Ech、Rnf)和各种生物合成途径的消失。不过,所有基因组中都保留了参与保护活性氧的基因(过氧化氢酶和超氧化物还原酶),包括细胞色素 bd(CydAB),这是一种高亲和性末端氧化酶,可能赋予了微需氧呼吸的能力。由于与宿主相关的甲烷弧菌嵌套在杂食性菌系中,我们得出结论,甲基的特化是对肠道环境的一种适应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome reduction in novel, obligately methyl-reducing Methanosarcinales isolated from arthropod guts (Methanolapillus gen. nov. and Methanimicrococcus).

Recent metagenomic studies have identified numerous lineages of hydrogen-dependent, obligately methyl-reducing methanogens. Yet, only a few representatives have been isolated in pure culture. Here, we describe six new species with this capability in the family Methanosarcinaceae (order Methanosarcinales), which makes up a substantial fraction of the methanogenic community in arthropod guts. Phylogenomic analysis placed the isolates from cockroach hindguts into the genus Methanimicrococcus (M. hacksteinii, M. hongohii, and M. stummii) and the isolates from millipede hindguts into a new genus, Methanolapillus (M. africanus, M. millepedarum, and M. ohkumae). Members of this intestinal clade, which includes also uncultured representatives from termites and vertebrates, have substantially smaller genomes (1.6-2.2 Mbp) than other Methanosarcinales. Genome reduction was accompanied by the loss of the upper part of the Wood-Ljungdahl pathway, several energy-converting membrane complexes (Fpo, Ech, and Rnf), and various biosynthetic pathways. However, genes involved in the protection against reactive oxygen species (catalase and superoxide reductase) were conserved in all genomes, including cytochrome bd (CydAB), a high-affinity terminal oxidase that may confer the capacity for microaerobic respiration. Since host-associated Methanosarcinales are nested within omnivorous lineages, we conclude that the specialization on methyl groups is an adaptation to the intestinal environment.

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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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