禾本科镰刀菌效应子 FgEC1 以小麦 TaGF14b 蛋白为靶标,抑制 TaRBOHD 介导的 ROS 生成并促进感染。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shengping Shang, Yuhan He, Qianyong Hu, Ying Fang, Shifeng Cheng, Cui-Jun Zhang
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引用次数: 0

摘要

由禾谷镰刀菌(Fusarium graminearum)引起的镰刀菌头枯病(FHB)是全球小麦的一种毁灭性病害。然而,禾谷镰孢与小麦之间相互作用的分子机制仍不清楚。在这里,我们发现了一种分泌型效应蛋白 FgEC1,它在小麦感染过程中被诱导,并且是禾谷镰刀菌毒力所必需的。FgEC1 可抑制 flg22 和几丁质诱导的胼胝质沉积以及烟草中的活性氧(ROS)爆发。FgEC1与TaGF14b直接相互作用,而TaGF14b在禾谷镰孢感染小麦时会在小麦头部上调。过表达 TaGF14b 可提高小麦的 FHB 抗性而不影响产量。TaGF14b 与 NADPH 氧化酶呼吸猝灭氧化酶同源物 D(TaRBOHD)相互作用,保护它不被 26S 蛋白酶体降解。FgEC1抑制了TaGF14b与TaRBOHD的相互作用,促进了TaRBOHD的降解,从而减少了TaRBOHD介导的ROS产生。我们的研究结果揭示了一种新的致病机制,即真菌病原体通过效应物减少 TaRBOHD 介导的 ROS 产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD-mediated ROS production and promote infection

Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD-mediated ROS production and promote infection

Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of wheat globally. However, the molecular mechanisms underlying the interactions between F. graminearum and wheat remain unclear. Here, we identified a secreted effector protein, FgEC1, that is induced during wheat infection and is required for F. graminearum virulence. FgEC1 suppressed flg22- and chitin-induced callose deposition and reactive oxygen species (ROS) burst in Nicotiana benthamiana. FgEC1 directly interacts with TaGF14b, which is upregulated in wheat heads during F. graminearum infection. Overexpression of TaGF14b increases FHB resistance in wheat without compromising yield. TaGF14b interacts with NADPH oxidase respiratory burst oxidase homolog D (TaRBOHD) and protects it against degradation by the 26S proteasome. FgEC1 inhibited the interaction of TaGF14b with TaRBOHD and promoted TaRBOHD degradation, thereby reducing TaRBOHD-mediated ROS production. Our findings reveal a novel pathogenic mechanism in which a fungal pathogen acts via an effector to reduce TaRBOHD-mediated ROS production.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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