基于壳聚糖的骨软骨构建体在玻璃体内的降解表明了对细胞活力的短暂影响。

Katherine Pitrolino, Reda Felfel, George Roberts, Colin Scotchford, David Grant, Virginie Sottile
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引用次数: 0

摘要

生物可吸收壳聚糖支架已显示出骨软骨修复应用的潜力。在溶菌酶的介导下,壳聚糖在体内降解,并释放出氨基葡萄糖,从而使生长组织逐渐取代壳聚糖。在此,研究人员对基于壳聚糖-NHA 的生物可吸收支架的降解过程进行了研究,以了解其在临床相关酶浓度作用下的质量损失、机械性能以及从支架中释放的降解产物。在降解的早期阶段,支架显示出加速的质量损失,但机械强度没有大幅降低,结构也没有恶化。虽然没有细胞毒性,但在支架降解超过两周的培养基中,间充质干细胞的存活率出现短暂下降,而主要降解产物(葡萄糖胺)在达到峰值浓度时,可能会对存活率产生不利影响。这项研究对壳聚糖支架的设计和生物医学应用具有重要意义,强调了模拟降解产物以确定是否适合临床应用的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitrodegradation of a chitosan-based osteochondral construct points to a transient effect on cellular viability.

Bioresorbable chitosan scaffolds have shown potential for osteochondral repair applications. Thein vivodegradation of chitosan, mediated by lysozyme and releasing glucosamine, enables progressive replacement by ingrowing tissue. Here the degradation process of a chitosan-nHA based bioresorbable scaffold was investigated for mass loss, mechanical properties and degradation products released from the scaffold when subjected to clinically relevant enzyme concentrations. The scaffold showed accelerated mass loss during the early stages of degradation but without substantial reduction in mechanical strength or structure deterioration. Although not cytotoxic, the medium in which the scaffold was degraded for over 2 weeks showed a transient decrease in mesenchymal stem cell viability, and the main degradation product (glucosamine) demonstrated a possible adverse effect on viability when added at its peak concentration. This study has implications for the design and biomedical application of chitosan scaffolds, underlining the importance of modelling degradation products to determine suitability for clinical translation.

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