开发 3D-iNET ORION:用于模拟人类胰腺转移性神经内分泌肿瘤的新型临床前三维体外细胞模型。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI:10.1007/s13577-024-01113-7
Jan Strnadel, Mark A Valasek, Grace Y Lin, Huahui Lin, Ann M Ponsford Tipps, Sang Myung Woo, Ken Fujimura, Huawei Wang, Sunkyu Choi, Jack Bui, Christopher Hermosillo, Kristen Jepsen, Michael R Navarro, Jonathan A Kelber, Richard L Klemke, Michael Bouvet
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引用次数: 0

摘要

胰腺神经内分泌肿瘤(NET)是一种罕见的肿瘤,由于其病程缓慢,给诊断和治疗带来了复杂的挑战。在过去二十年中,胰腺神经内分泌肿瘤的发病率显著增加。目前可用于研究的胰腺神经内分泌细胞系数量有限。在此,我们介绍一种新型三维(球形)细胞系--3D-iNET ORION,它是从人类胰腺神经内分泌肿瘤肝转移瘤中分离出来的。三维生长(3D)肿瘤细胞系能更好地再现肿瘤的体内结构,更适合体外和体内实验,因此在过去几年中备受关注。3D-iNET ORION 癌细胞系在嵌入 Matrigel 基质后显示出形成瘤球的高潜力,并表达突触素和 EpCAM。癌细胞系的电子显微镜分析表明存在致密的神经分泌颗粒。当异种移植到无胸腺小鼠体内时,3D-iNET ORION 细胞会产生生长缓慢的肿瘤,嗜铬粒蛋白和突触素呈阳性。人类核心外显子组分析显示,3DiNET ORION 细胞系保留了原始肿瘤中检测到的基因畸变特征。总之,我们新开发的神经内分泌癌细胞系可作为一种新的研究工具,用于体外和体内实验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of 3D-iNET ORION: a novel, pre-clinical, three-dimensional in vitro cell model for modeling human metastatic neuroendocrine tumor of the pancreas.

Development of 3D-iNET ORION: a novel, pre-clinical, three-dimensional in vitro cell model for modeling human metastatic neuroendocrine tumor of the pancreas.

Neuroendocrine tumors (NETs) of the pancreas are rare neoplasms that present complex challenges to diagnosis and treatment due to their indolent course. The incidence of pancreatic neuroendocrine tumors has increased significantly over the past two decades. A limited number of pancreatic neuroendocrine cell lines are currently available for the research. Here, we present 3D-iNET ORION, a novel 3-dimensional (spheroid) cell line, isolated from human pancreatic neuroendocrine tumor liver metastasis. Three-dimensionally grown (3D) cancer cell lines have gained interest over the past years as 3D cancer cell lines better recapitulate the in vivo structure of tumors, and are more suitable for in vitro and in vivo experiments. 3D-iNET ORION cancer cell line showed high potential to form tumorspheres when embedded in Matrigel matrix and expresses synaptophysin and EpCAM. Electron microscopy analysis of cancer cell line proved the presence of dense neurosecretory granules. When xenografted into athymic mice, 3D-iNET ORION cells produce slow-growing tumors, positive for chromogranin and synaptophysin. Human Core Exome Panel Analysis has shown that 3DiNET ORION cell line retains the genetic aberration profile detected in the original tumor. In conclusion, our newly developed neuroendocrine cancer cell line can be considered as a new research tool for in vitro and in vivo experiments.

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CiteScore
7.20
自引率
4.30%
发文量
567
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